Kwon Hyeok-Tae, Lee Younmi, Kim Jungyeon, Balaraju Kotnala, Kim Heung Tae, Jeon Yongho
Department of Plant Medicals, Andong National University, Andong, South Korea.
Agricultural Science and Technology Research Institute, Andong National University, Andong, South Korea.
Front Microbiol. 2022 Mar 2;13:826827. doi: 10.3389/fmicb.2022.826827. eCollection 2022.
Anthracnose is a fungal disease caused by species and has detrimental effects on many crops, including red pepper. This study used GYUN-300 (GYUN-300), which exhibit antagonistic activity against the fungal pathogen, . This pathogen causes anthracnose that manifests primarily as a fruit rot in red pepper. There have been little efforts to identify antagonistic bacteria from mushrooms; this strain of bacteria was identified as using BIOLOG and 16S rDNA sequencing analysis. The genetic mechanism underpinning the biocontrol traits of GYUN-300 was characterized using the complete genome sequence of GYUN-300, which was closely compared to related strains. GYUN-300 inhibited mycelial growth and spore germination of under conditions. Important antagonistic traits, such as siderophore production, solubilization of insoluble phosphate, and production of lytic enzymes (cellulase, protease, and amylase), were observed in GYUN-300, These trains promoted growth in terms of seed germination and vigorous seedling growth compared to the non-treated control. When red pepper fruits were treated with GYUN-300, the preventive and curative effects were 66.6 and 38.3% effective, respectively, in wounded red pepper fruits; there was no difference between the preventive and curative effects in non-wounded red pepper fruits. Furthermore, GYUN-300 was resistant to several commercial fungicides, indicating that GYUN-300 bacterial cells may also be used synergistically with chemical fungicides to increase biocontrol efficiency. Based on results, GYUN-300 played a role to control anthracnose disease effectively in field conditions when compared to other treatments and non-treated controls. The results from this study provide a better understanding of the GYUN-300 strain as an effective biocontrol agent against red pepper anthracnose; this form of biocontrol provides an environment-friendly alternative to chemical fungicides.
炭疽病是由多种物种引起的一种真菌病害，对包括红辣椒在内的许多作物都有不利影响。本研究使用了GYUN - 300（GYUN - 300），它对真菌病原体表现出拮抗活性。这种病原体导致炭疽病，在红辣椒中主要表现为果实腐烂。从蘑菇中鉴定拮抗细菌的工作很少；通过BIOLOG和16S rDNA测序分析，该菌株被鉴定为[具体菌种未给出]。利用GYUN - 300的全基因组序列对其生物防治特性的遗传机制进行了表征，并与相关菌株进行了密切比较。在[具体条件未给出]条件下，GYUN - 300抑制了[具体病原体未给出]的菌丝生长和孢子萌发。在GYUN - 300中观察到了重要的拮抗特性，如铁载体的产生、不溶性磷酸盐的溶解以及裂解酶（纤维素酶、蛋白酶和淀粉酶）的产生。与未处理的对照相比，这些特性促进了种子萌发和幼苗茁壮成长。用GYUN - 300处理红辣椒果实后，在受伤的红辣椒果实中，预防和治疗效果分别为66.6%和38.3%有效；在未受伤的红辣椒果实中，预防和治疗效果没有差异。此外，GYUN - 300对几种商业杀菌剂具有抗性，这表明GYUN - 300细菌细胞也可与化学杀菌剂协同使用，以提高生物防治效率。基于[具体结果未给出]结果，与其他处理和未处理的对照相比，GYUN - 300在田间条件下有效地控制了炭疽病。本研究结果有助于更好地理解GYUN - 300菌株作为防治红辣椒炭疽病的有效生物防治剂；这种生物防治形式为化学杀菌剂提供了一种环境友好的替代方案。
