Chen Meixia, Lin Hui, Zu Weifan, Wang Lulu, Dai Wenbo, Xiao Yulin, Zou Ye, Zhang Chengkang, Liu Wei, Niu Xiaoping
College of Biological Science and Engineering, Industry and University Research Cooperation Demonstration Base in Fujian Province, Ningde Normal University, Ningde 352100, China.
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel). 2024 Oct 15;13(20):2889. doi: 10.3390/plants13202889.
Anthracnose of the tea plant (), caused by spp., poses a significant threat to both the yield and quality of tea production. To address this challenge, researchers have looked to the application of endophytic bacteria as a natural alternative to the use chemical pesticides, offering potential for enhancing disease resistance and abiotic stress tolerance in tea plants. This study focused on identifying effective microbial agents to combat tea anthracnose caused by . A total of 38 -like strains were isolated from the tea rhizosphere, with 8 isolates showing substantial inhibitory effects against the mycelial growth of , achieving an average inhibition rate of 60.68%. Among these, strain T3 was particularly effective, with a 69.86% inhibition rate. Through morphological, physiological, and biochemical characterization, along with 16S rRNA gene phylogenetics analysis, these strains were identified as (T1 and T2), (T3, T5, T7, T8, and T19), and (T6). Biological and molecular assays confirmed that these strains could induce the expression of genes associated with antimicrobial compounds like iturin, fengycin, subtilosin, and alkaline protease, which effectively reduced the disease index of tea anthracnose and enhanced tea plant growth. In conclusion, this study demonstrates that , , and strains are promising biocontrol agents for managing tea anthracnose.
茶树炭疽病（）由 种引起，对茶叶生产的产量和质量都构成了重大威胁。为应对这一挑战，研究人员将内生细菌的应用视为替代化学农药的天然选择，有望增强茶树的抗病性和非生物胁迫耐受性。本研究着重于鉴定对抗由 引起的茶树炭疽病的有效微生物制剂。从茶根际共分离出38株类似的菌株，其中8株对 的菌丝生长表现出显著抑制作用，平均抑制率达60.68%。其中，菌株T3尤为有效，抑制率为69.86%。通过形态学、生理学和生化特征鉴定，以及16S rRNA基因系统发育分析，这些菌株被鉴定为 （T1和T2）、 （T3、T5、T7、T8和T19）和 （T6）。生物学和分子分析证实，这些菌株可诱导与抗微生物化合物如伊枯草菌素、丰原素、枯草芽孢杆菌素和碱性蛋白酶相关的基因表达，有效降低茶树炭疽病的病情指数并促进茶树生长。总之，本研究表明 、 和 菌株是防治茶树炭疽病的有前景的生物防治剂。
