Palmer Bradley M, Bell Stephen P
Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT, United States.
Department of Medicine, University of Vermont, Burlington, VT, United States.
Front Physiol. 2022 Mar 3;13:817205. doi: 10.3389/fphys.2022.817205. eCollection 2022.
While the reductionist approach has been fruitful in understanding the molecular basis of muscle function, intact excitable muscle preparations are still important as experimental model systems. We present here methods that are useful for preparing cardiac papillary muscle and cardiac slices, which represent macroscopic experimental model systems with fully intact intercellular and intracellular structures. The maintenance of these structures for experimentation have made these model systems especially useful for testing the functional effects of protein mutations and pharmaceutical candidates. We provide solutions recipes for dissection and recording, instructions for removing and preparing the cardiac papillary muscles, as well as instruction for preparing cardiac slices. These instructions are suitable for beginning experimentalists but may be useful for veteran muscle physiologists hoping to reacquaint themselves with macroscopic functional analyses.
虽然还原论方法在理解肌肉功能的分子基础方面卓有成效，但完整的可兴奋肌肉制剂作为实验模型系统仍然很重要。我们在此介绍一些对制备心脏乳头肌和心脏切片有用的方法，这些代表了具有完全完整细胞间和细胞内结构的宏观实验模型系统。维持这些用于实验的结构使这些模型系统对于测试蛋白质突变和候选药物的功能效应特别有用。我们提供了解剖和记录的溶液配方、去除和制备心脏乳头肌的说明，以及制备心脏切片的说明。这些说明适合刚开始做实验的人员，但对于希望重新熟悉宏观功能分析的资深肌肉生理学家可能也有用。
