评估致病变异对小鼠大脑形态和原代海马神经元影响的方案。
Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons.
机构信息
Department of Genetics and Development, Columbia University Irving Medical Center, New York, NY 10032, USA.
Department of Ophthalmology, Columbia University Irving Medical Center, New York, NY 10032, USA.
出版信息
STAR Protoc. 2022 Mar 15;3(2):101244. doi: 10.1016/j.xpro.2022.101244. eCollection 2022 Jun 17.
Genetic variants that affect neurological function will often produce changes visible at the level of gross morphology, either of the whole brain or of specific neuronal types. Here we describe how to perfuse and dissect the brain in preparation for Nissl staining. Then we outline steps for culturing mouse primary hippocampal neurons to evaluate dendritic arborization (Sholl analysis). For complete details on the use and execution of this protocol, please refer to Gennarino et al. (2018).
影响神经功能的遗传变异通常会导致大脑整体形态或特定神经元类型的宏观形态上可见的变化。在这里，我们描述了如何进行灌注和解剖大脑，以便进行尼氏染色。然后，我们概述了培养小鼠原代海马神经元以评估树突分支（Sholl 分析）的步骤。有关该方案使用和执行的完整详细信息，请参见 Gennarino 等人（2018 年）。
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