通过超高分辨率显微镜定量分析来自小鼠海马神经元的兴奋性突触中的分子聚集。

Centre for Neuroscience, Indian Institute of Science, Bangalore 560012, India.

STAR Protoc. 2021 Apr 14;2(2):100470. doi: 10.1016/j.xpro.2021.100470. eCollection 2021 Jun 18.

Super-resolution microscopy (SRM) has been widely adopted to probe molecular distribution at excitatory synapses. We present an SRM paradigm to evaluate the nanoscale organization heterogeneity between neuronal subcompartments. Using mouse hippocampal neurons, we describe the identification of the morphological characteristics of nanodomains within functional zones of a single excitatory synapse. This information can be used to correlate structure and function at molecular resolution in single synapses. The protocol can be applied to immunocytochemical/histochemical samples across different imaging paradigms. For complete details on the use and execution of this protocol, please refer to Kedia et al. (2021).

超分辨率显微镜（SRM）已被广泛用于探测兴奋性突触处的分子分布。我们提出了一种 SRM 范例，以评估神经元亚区之间纳米尺度组织异质性。使用小鼠海马神经元，我们描述了在单个兴奋性突触的功能区中纳米结构域的形态特征的识别。此信息可用于在单个突触中以分子分辨率关联结构和功能。该方案可应用于跨不同成像范例的免疫细胞化学/组织化学样本。有关此方案使用和执行的完整详细信息，请参阅 Kedia 等人。（2021）。