Aguirre P, Scully R E, Wolfe H J, DeLellis R A
Int J Gynecol Pathol. 1986;5(3):223-34. doi: 10.1097/00004347-198609000-00004.
Argyrophil cells were identified by the single-impregnation Grimelius technique in 11 of 28 (39%) Brenner tumors, accounting for less than 1% of the tumor cell population in all the cases. All tumors with argyrophil cells were stained to demonstrate calcitonin, somatostatin, gastrin, adrenocorticotropic hormone, neurotensin, insulin, glucagon, and serotonin; and four of them (three benign and one borderline) were also stained for chromogranins with the monoclonal antibody LK2H10. Serotonin was present in nine of the 11 cases with argyrophil cells. Neurotensin and somatostatin were found in one borderline tumor, which also contained serotonin. Chromogranin reactivity was demonstrated in all four cases in which it was examined. Ultrastructural examination of one tumor revealed that the argyrophil cells contained secretory granules, 80 nm in diameter, and had elongated cytoplasmic processes that extended between the more numerous nonargyrophil tumor cells. The argyrophil cells of Brenner tumors are similar to those of urothelium in the frequency with which they are immunoreactive for serotonin and the rarity with which they are reactive for peptide hormones. These cells differ from those of mucinous ovarian tumors, which often contain both serotonin and peptide hormones. The findings of this study lend additional support to the close similarity of the epithelial components of Brenner tumors and urothelium.
采用单浸染Grimelius技术，在28例Brenner瘤中的11例（39%）中鉴定出亲银细胞，在所有病例中亲银细胞占肿瘤细胞总数不到1%。所有含有亲银细胞的肿瘤均进行染色以显示降钙素、生长抑素、胃泌素、促肾上腺皮质激素、神经降压素、胰岛素、胰高血糖素和血清素；其中4例（3例良性和1例交界性）还用单克隆抗体LK2H10进行嗜铬粒蛋白染色。11例有亲银细胞的病例中有9例存在血清素。在1例交界性肿瘤中发现了神经降压素和生长抑素，该肿瘤也含有血清素。在所检查的4例病例中均显示有嗜铬粒蛋白反应性。对1例肿瘤的超微结构检查显示，亲银细胞含有直径80nm的分泌颗粒，并有细长的胞质突起，延伸于数量较多的非亲银性肿瘤细胞之间。Brenner瘤的亲银细胞与尿路上皮的亲银细胞在血清素免疫反应频率和肽类激素反应罕见性方面相似。这些细胞与黏液性卵巢肿瘤的细胞不同，后者通常同时含有血清素和肽类激素。本研究结果进一步支持了Brenner瘤的上皮成分与尿路上皮密切相似的观点。
