Vasquez-Rifo Alejandro, Cook Jamie, McEwan Deborah L, Shikara Dania, Ausubel Frederick M, Di Cara Francesca, Cheng Zhenyu
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Department of Microbiology and Immunology, Dalhousie Universitygrid.55602.34, Halifax, Nova Scotia, Canada.
mBio. 2022 Apr 26;13(2):e0041722. doi: 10.1128/mbio.00417-22. Epub 2022 Mar 21.
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that exhibits pathogenicity in an unusually broad range of plants and animals, and it is of interest to study the roles of particular virulence-related factors in diverse hosts. The production of many P. aeruginosa virulence factors is under the control of a quorum sensing (QS) signaling network, which has three interconnected branches that engage in intricate cross talk: Las, Rhl, and MvfR. Because there has been no systematic comparison of the roles of the three QS systems in mediating P. aeruginosa virulence in various hosts, we compared the virulence of wild-type (WT) P. aeruginosa PA14 and a set of isogenic PA14 QS in-frame deletion mutants in four selected hosts, the reference plant Arabidopsis thaliana (Arabidopsis), the crop plant Brassica napus (canola), the nematode Caenorhabditis elegans, and the fruit fly Drosophila melanogaster. The first letters of the selected host genera, A, B, C, and D, inspired the title of this article and indicate that this work lays the groundwork for future elucidation of the specific roles of each QS branch in mediating virulence in diverse hosts. In this study, we performed a systematic comparison of the virulence of WT P. aeruginosa and QS mutants in selected hosts and conditions. This work represents an important contribution to the long-term goal of unraveling the entangled roles of different branches of the P. aeruginosa QS network in different hosts and will serve as a valuable resource for the field of host-pathogen interactions.
铜绿假单胞菌是一种机会性细菌病原体，在异常广泛的动植物中表现出致病性，研究特定毒力相关因子在不同宿主中的作用很有意义。许多铜绿假单胞菌毒力因子的产生受群体感应（QS）信号网络控制，该网络有三个相互连接的分支，它们之间存在复杂的相互作用：Las、Rhl和MvfR。由于尚未对这三个QS系统在介导铜绿假单胞菌在各种宿主中的毒力方面的作用进行系统比较，我们比较了野生型（WT）铜绿假单胞菌PA14和一组同基因PA14 QS框内缺失突变体在四个选定宿主中的毒力，即参考植物拟南芥、农作物甘蓝型油菜（油菜）、线虫秀丽隐杆线虫和果蝇黑腹果蝇。选定宿主属的首字母A、B、C和D启发了本文的标题，并表明这项工作为未来阐明每个QS分支在介导不同宿主中毒力的具体作用奠定了基础。在本研究中，我们对野生型铜绿假单胞菌和QS突变体在选定宿主和条件下的毒力进行了系统比较。这项工作对解开铜绿假单胞菌QS网络不同分支在不同宿主中的复杂作用这一长期目标做出了重要贡献，并将成为宿主-病原体相互作用领域的宝贵资源。
