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dnaK和dnaJ基因产物参与大肠杆菌K-12谷氨酰胺-tRNA合成酶和苏氨酸-tRNA合成酶的磷酸化作用。

Participation of the dnaK and dnaJ gene products in phosphorylation of glutaminyl-tRNA synthetase and threonyl-tRNA synthetase of Escherichia coli K-12.

作者信息

Wada M, Sekine K, Itikawa H

出版信息

J Bacteriol. 1986 Oct;168(1):213-20. doi: 10.1128/jb.168.1.213-220.1986.

DOI:10.1128/jb.168.1.213-220.1986
PMID:3531168
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC213440/
Abstract

The heat shock proteins DnaK and DnaJ of Escherichia coli participate in phosphorylation of both glutaminyl-tRNA synthetase and threonyl-tRNA synthetase. When cellular proteins extracted from the dnaK7(Ts) and dnaJ259(Ts) mutant cells labeled with 32Pi at 42 degrees C were analyzed by two-dimensional gel electrophoresis, no phosphorylation of these proteins was observed when they were compared with those from wild-type cells.

摘要

大肠杆菌的热休克蛋白DnaK和DnaJ参与谷氨酰胺-tRNA合成酶和苏氨酸-tRNA合成酶的磷酸化过程。当对在42℃用32P i标记的dnaK7（Ts）和dnaJ259（Ts）突变细胞中提取的细胞蛋白进行二维凝胶电泳分析时，与野生型细胞的蛋白相比，未观察到这些蛋白的磷酸化现象。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/9bc36e7f9b4e/jbacter00203-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/e8a44188d987/jbacter00203-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/c6064734ca88/jbacter00203-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/5e3b3ace9270/jbacter00203-0225-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/cba22be9edbc/jbacter00203-0226-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/3053547a4068/jbacter00203-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/b7fd809b8e75/jbacter00203-0227-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/da359a42154c/jbacter00203-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/497525b2facf/jbacter00203-0228-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/9bc36e7f9b4e/jbacter00203-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/e8a44188d987/jbacter00203-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/c6064734ca88/jbacter00203-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/5e3b3ace9270/jbacter00203-0225-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/cba22be9edbc/jbacter00203-0226-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/3053547a4068/jbacter00203-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/b7fd809b8e75/jbacter00203-0227-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/da359a42154c/jbacter00203-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/497525b2facf/jbacter00203-0228-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c3/213440/9bc36e7f9b4e/jbacter00203-0229-a.jpg

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本文引用的文献

1
The genetics and regulation of heat-shock proteins.热休克蛋白的遗传学与调控
Annu Rev Genet. 1984;18:295-329. doi: 10.1146/annurev.ge.18.120184.001455.
2
Threonyl-tRNA synthetase from Chinese hamster ovary cells is phosphorylated on serine.来自中国仓鼠卵巢细胞的苏氨酰-tRNA合成酶在丝氨酸上被磷酸化。
J Biol Chem. 1984 Sep 25;259(18):11160-1.
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Protein phosphorylation in Escherichia coli and purification of a protein kinase.
J Biol Chem. 1984 Jan 10;259(1):526-33.
大肠杆菌K-12 RNA聚合酶一个改变的亚基对温度敏感型groES突变的遗传抑制作用。
J Bacteriol. 1987 Mar;169(3):1102-6. doi: 10.1128/jb.169.3.1102-1106.1987.
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Suppression of mutations conferring penicillin tolerance by interference with the stringent control mechanism of Escherichia coli.通过干扰大肠杆菌的严谨控制机制来抑制赋予青霉素耐受性的突变。
J Bacteriol. 1987 Sep;169(9):4396-8. doi: 10.1128/jb.169.9.4396-4398.1987.
5
High levels of manganese-containing superoxide dismutase and thermally induced DNA disruption in a dnaK7(Ts) mutant of Escherichia coli K12.大肠杆菌K12的dnaK7（Ts）突变体中高水平的含锰超氧化物歧化酶和热诱导的DNA破坏
Mol Gen Genet. 1989 Jun;217(2-3):317-23. doi: 10.1007/BF02464899.
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Cellular defects caused by deletion of the Escherichia coli dnaK gene indicate roles for heat shock protein in normal metabolism.大肠杆菌dnaK基因缺失导致的细胞缺陷表明热休克蛋白在正常代谢中的作用。
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7
Protein phosphorylation in response to stress in Clostridium acetobutylicum.丙酮丁醇梭菌中应激反应下的蛋白质磷酸化
Appl Environ Microbiol. 1990 Jul;56(7):2170-3. doi: 10.1128/aem.56.7.2170-2173.1990.
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Identification and characterization of a new Escherichia coli gene that is a dosage-dependent suppressor of a dnaK deletion mutation.
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Bacteriol Rev. 1966 Dec;30(4):701-19. doi: 10.1128/br.30.4.701-719.1966.