Wada M, Itikawa H
J Bacteriol. 1984 Feb;157(2):694-6. doi: 10.1128/jb.157.2.694-696.1984.
Cells having the temperature-sensitive mutation groES131(Ts) were isolated from Escherichia coli K-12 strain C600T by thymineless death selection at 44 degrees C. This conditionally expressed mutation affected both cellular DNA and RNA syntheses at nonpermissive temperature, in addition to rendering cells unable to propagate phage lambda at permissive temperature.
通过在44℃下进行无胸腺死亡选择,从大肠杆菌K-12菌株C600T中分离出具有温度敏感突变groES131(Ts)的细胞。这种条件表达的突变在非允许温度下影响细胞DNA和RNA的合成,此外,在允许温度下使细胞无法繁殖噬菌体λ。