Gerken S C, Arfin S M
J Biol Chem. 1984 Sep 25;259(18):11160-1.
A Chinese hamster ovary cell line, 2000A, in which threonyl-tRNA synthetase accounts for 1.5% of the total soluble protein, was used to demonstrate that this enzyme is a phosphoprotein. Threonyl-tRNA synthetase was isolated by immunoprecipitation from cells labeled with 32Pi for 18 h. Phosphoamino acid analysis of radiolabeled threonyl-tRNA synthetase showed that phosphorylation occurs on serine.
用一种中国仓鼠卵巢细胞系2000A来证明苏氨酰 - tRNA合成酶是一种磷蛋白，在该细胞系中，苏氨酰 - tRNA合成酶占总可溶性蛋白的1.5%。通过免疫沉淀从用32P i标记18小时的细胞中分离出苏氨酰 - tRNA合成酶。对放射性标记的苏氨酰 - tRNA合成酶进行磷酸氨基酸分析表明，磷酸化发生在丝氨酸上。
