鼠伤寒沙门氏菌cysB区域的克隆与物理图谱构建。
Cloning and physical mapping of the cysB region of Salmonella typhimurium.
作者信息
Jagura-Burdzy G, Kredich N M
出版信息
J Bacteriol. 1983 Aug;155(2):578-85. doi: 10.1128/jb.155.2.578-585.1983.
Abstract
The cysB region of Salmonella typhimurium was cloned in pBR322 and localized to a 1.75-kilobase HincII fragment. Two-dimensional protein electropherograms showed levels of the cysB polypeptide chain that were several fold higher in plasmid-bearing strains than in the wild type. Fully derepressed levels of sulfite reductase and O-acetylserine sulfhydrylase in cysB plasmid-bearing strains were only 25% higher than in the wild type, suggesting that the product of this regulatory gene ordinarily is not a limiting factor in the expression of the cysteine regulon. The mapping of cysB deletions by Southern blots showed a good correlation between the genetic and the physical maps of this gene. The supX gene was initially cloned with cysB and is within 0.7 kilobase of cysB.
摘要
鼠伤寒沙门氏菌的cysB区域被克隆到pBR322中，并定位在一个1.75千碱基的HincII片段上。二维蛋白质电泳图谱显示，携带质粒的菌株中cysB多肽链的水平比野生型高几倍。携带cysB质粒的菌株中亚硫酸盐还原酶和O-乙酰丝氨酸巯基酶的完全去阻遏水平仅比野生型高25%，这表明该调节基因的产物通常不是半胱氨酸调节子表达中的限制因素。通过Southern印迹法对cysB缺失进行定位，结果表明该基因的遗传图谱和物理图谱之间具有良好的相关性。supX基因最初与cysB一起被克隆，且位于cysB的0.7千碱基范围内。
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