从麦芽糖假丝酵母基因组中克隆出一个修饰核糖体的环己酰亚胺抗性基因，该基因来自酿酒酵母。
Cloning in Saccharomyces cerevisiae of a cycloheximide resistance gene from the Candida maltosa genome which modifies ribosomes.
作者信息
Takagi M, Kawai S, Shibuya I, Miyazaki M, Yano K
出版信息
J Bacteriol. 1986 Oct;168(1):417-9. doi: 10.1128/jb.168.1.417-419.1986.
Abstract
We have previously shown that cycloheximide resistance can be induced in a strain of Candida maltosa by modifying ribosomes (M. Takagi, S. Kawai, Y. Takata, N. Tanaka, M. Sunairi, M. Miyazaki, and K. Yano, J. Gen. Appl. Microbiol. 31:267-275, 1985). The present paper describes the cloning of the gene involved in this resistance (designated RIM-C for ribosome modification by cycloheximide) by using a host-vector system of Saccharomyces cerevisiae.
摘要
我们之前已经表明，通过修饰核糖体，在麦芽糖假丝酵母菌株中可诱导出对放线菌酮的抗性（M. 高木、S. 川井、Y. 高田、N. 田中、M. 须奈里、M. 宫崎和K. 矢野，《普通与应用微生物学杂志》31:267 - 275，1985年）。本文描述了利用酿酒酵母宿主 - 载体系统克隆参与这种抗性的基因（命名为RIM - C，即通过放线菌酮进行核糖体修饰）。
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Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
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