甘草酸诱导肝癌细胞 G 期阻滞与 DNA 损伤和 NF-κB 失活有关。
DNA damage and NF-κB inactivation implicate glycyrrhizic acid-induced G phase arrest in hepatocellular carcinoma cells.
机构信息
Department of Medicine, National Yang Ming Chiao Tung University Hospital, Yilan, Taiwan.
School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.
出版信息
J Food Biochem. 2022 Jul;46(7):e14128. doi: 10.1111/jfbc.14128. Epub 2022 Mar 21.
Hepatocellular carcinoma (HCC) is the most frequently occurring liver malignancy in Asia. Glycyrrhizic acid is known to reduce the risk of HCC formation in patients with chronic hepatitis C. To identify whether glycyrrhizic acid may play a role in anti-HCC therapy as an adjuvant is important. However, the inhibitory effect of glycyrrhizic acid on cell cycle progression in HCC cells and the mechanism of such have not been fully elucidated. This study used the comet assay, cell cycle analysis, immunofluorescence staining, the TUNEL assay, and Western blotting to identify the anti-HCC role of glycyrrhizic acid. Glycyrrhizic acid may induce DNA damage, apoptosis, activation of ATM, and expression of p21, and p27 in HCC cells. In addition, glycyrrhizic acid may also induce G phase arrest and suppress NF-κB-mediated Cyclin D1 expression. DNA damage and NF-κB inactivation may be associated with glycyrrhizic acid-induced G phase arrest in HCC cells.
肝细胞癌（HCC）是亚洲最常见的肝脏恶性肿瘤。甘草酸已被证实可降低慢性丙型肝炎患者 HCC 形成的风险。确定甘草酸是否可作为辅助药物在抗 HCC 治疗中发挥作用非常重要。然而，甘草酸对 HCC 细胞周期进展的抑制作用及其机制尚未完全阐明。本研究采用彗星试验、细胞周期分析、免疫荧光染色、TUNEL 检测和 Western blot 分析来确定甘草酸的抗 HCC 作用。甘草酸可能诱导 HCC 细胞中的 DNA 损伤、凋亡、ATM 激活以及 p21 和 p27 的表达。此外，甘草酸还可能诱导 G 期阻滞并抑制 NF-κB 介导的 Cyclin D1 表达。DNA 损伤和 NF-κB 失活可能与甘草酸诱导 HCC 细胞 G 期阻滞有关。
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