Université de Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 69100, Villeurbanne, France.
Université Clermont Auvergne, Imagerie Moléculaire et Stratégies Théranostiques, Inserm, U 1240, Centre Jean Perrin BP 184, F-63011, Clermont-Ferrand, France.
Talanta. 2022 Jun 1;243:123386. doi: 10.1016/j.talanta.2022.123386. Epub 2022 Mar 16.
Despite the tremendous interest for nanoparticles (NPs) in the biomedical field, their transfer to the clinics is still hampered, in particular due to the lack of knowledge of their behaviour in a biological environment. Indeed, the protein corona formed as soon as NPs enter the bloodstream can drastically affect their properties. The use of Taylor dispersion analysis-ICP-MS as an efficient technique dedicated to metal-containing NPs was proposed to examine these NP-protein interactions and determine protein corona thicknesses in biological fluids. This method was applied on core-shell gold/silica NPs in the presence of proteins at high concentrations and serum. Protein corona around 4 nm were measured. Moreover, the versatility of the method allowed assessing the reversible/irreversible character of the interactions.
尽管纳米颗粒 (NPs) 在生物医学领域引起了极大的兴趣，但它们向临床的转移仍然受到阻碍，特别是由于缺乏对其在生物环境中的行为的了解。事实上，纳米颗粒一旦进入血液，就会形成蛋白质冠，这会极大地影响它们的性质。本文提出了使用泰勒分散分析-ICP-MS 作为一种专门用于含金属 NPs 的有效技术，以研究这些 NP-蛋白质相互作用并确定生物流体中的蛋白质冠厚度。该方法应用于高浓度蛋白质和血清存在下的核壳金/硅纳米颗粒。测量了约 4nm 的蛋白质冠。此外，该方法的多功能性允许评估相互作用的可逆/不可逆性质。
