Mekhalif Fatima, Zgheib Rita, Akiana Jean, Bilen Melhem, Ndombe Geor Mongo, Fenollar Florence, Fournier Pierre-Edouard, Raoult Didier, Alibar Stéphane, Mediannikov Oleg, Lo Cheikh Ibrahima
Aix Marseille Univ, IRD, AP-HM, MEPHI, Marseille, France.
IHU-Méditerranée Infection, Marseille, France.
Heliyon. 2022 Mar 14;8(3):e09102. doi: 10.1016/j.heliyon.2022.e09102. eCollection 2022 Mar.
The Microbial Culturomics Project aiming to discover several bacterial species made it possible to isolate the strain Marseille-P4308 from a stool sample of a healthy indigenous Congolese volunteer. Strain Marseille-P4308 is a Gram-positive coccus shaped bacterium that optimally grows at 37 °C. The 16S rRNA gene sequence of the strain has a 96.2% sequence similarity to strain NCTC 11460 (GenBank accession number: NR_042847.1). In addition, the average nucleotide identity of strain Marseille-P4308 with its closest related species was 71.1%, which was far below the recommended threshold (>95-96%). The genome of the strain Marseille-P4308 has a length of 2.14 Mbp with G + C content of 30.4 mol%. Based on phenotypic, biochemical, genomic and phylogenetic analysis, strain Marseille-P4308 (= CSUR P4308 = CECT 9960) clearly appears to be a new species for which the name sp. nov., is proposed.
旨在发现多种细菌物种的微生物培养组学项目使得从一名健康的刚果本土志愿者的粪便样本中分离出马赛 - P4308菌株成为可能。马赛 - P4308菌株是一种革兰氏阳性球菌,在37°C时生长最佳。该菌株的16S rRNA基因序列与NCTC 11460菌株(GenBank登录号:NR_042847.1)具有96.2%的序列相似性。此外,马赛 - P4308菌株与其最密切相关物种的平均核苷酸同一性为71.1%,远低于推荐阈值(>95 - 96%)。马赛 - P4308菌株的基因组长度为2.14 Mbp,G + C含量为30.4 mol%。基于表型、生化、基因组和系统发育分析,马赛 - P4308菌株(= CSUR P4308 = CECT 9960)显然是一个新物种,为此提出新物种名称sp. nov.。