Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan1, Ganjingzi District, Dalian 116034, Liaoning, China.
National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
Food Funct. 2022 Apr 4;13(7):4023-4031. doi: 10.1039/d1fo04222j.
Nanocarriers provide the possibility to overcome the low solubility, poor stability, and low bioavailability of functional factors. However, most nanocarriers do not directly participate in the corresponding effects of functional factors, such as treating inflammatory bowel disease but lack the means to control their size accurately. Herein, nanocarriers were prepared by a one-pot method, using food-grade antioxidant procyanidins, vanillin, and phycocyanin as raw materials. The strategy involved the Mannich reaction among the phenolic hydroxyl groups of procyanidins, the aldehyde groups of vanillin, and the amino groups of phycocyanin. The obtained nanocarriers displayed controllable sizes ranging from 130 to 750 nm, showing good antioxidant capacity in scavenging free radicals and were biocompatible to Caco-2 cells and RAW 264.7 macrophages. Nanocarriers also exhibited an inhibitory effect on cell damage induced by acrylamide and HO. Moreover, the designed nanocarriers could be used for delivering active ingredients such as lutein, which showed a uniform spherical distribution, high encapsulation efficiency, and good biocompatibility. This work provides a facile synthesis method to prepare food-grade nanocarriers with functional properties, which can be potentially used in the delivery of functional factors.
纳米载体提供了克服功能因子低溶解度、差稳定性和低生物利用度的可能性。然而，大多数纳米载体并不直接参与功能因子的相应作用，例如治疗炎症性肠病，但缺乏精确控制其大小的手段。在此，采用一锅法制备了纳米载体，以食品级抗氧化剂原花青素、香草醛和藻蓝蛋白为原料。该策略涉及原花青素酚羟基、香草醛醛基和藻蓝蛋白氨基之间的曼尼希反应。所得纳米载体的粒径可控，范围为 130 至 750nm，在清除自由基方面表现出良好的抗氧化能力，且对 Caco-2 细胞和 RAW 264.7 巨噬细胞具有生物相容性。纳米载体还对丙烯酰胺和 HO 诱导的细胞损伤表现出抑制作用。此外，设计的纳米载体可用于递送叶黄素等活性成分，表现出均匀的球形分布、高包封效率和良好的生物相容性。这项工作提供了一种简便的合成方法来制备具有功能特性的食品级纳米载体，可潜在用于功能因子的递送。
