State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, Liaoning, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China.
Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt; Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum 25240, Turkey.
Int J Biol Macromol. 2023 Dec 31;253(Pt 4):127039. doi: 10.1016/j.ijbiomac.2023.127039. Epub 2023 Sep 22.
Liver cancer, a malignancy with a rising global incidence, poses a significant challenge in achieving effective treatment outcomes. As food-derived nutrient, sea cucumber peptide (SCP) has shown promising anticancer effects. Therefore, we explored the nanodelivery systems to encapsulate SCP to enhance its stability in the gastrointestinal tract and improve absorption within the tumor microenvironment. This study aimed to develop size-controllable multifunctional nanoparticles using SCP, procyanidins (PCs), and vanillin through molecular assembly via a one-pot Mannich condensation approach. These food-grade nanoparticles demonstrated water solubility and exhibited a spherical structure with sizes ranging from 441 to 1360 nm, depending on the concentration of the reactants. In vitro cell experiments demonstrated that SCP nanoparticles modified with PCs effectively reduced the generation of reactive oxygen species from HO and acrylamide while maintaining normal levels of mitochondrial membrane potential. Furthermore, in vivo nutrition intervention studies conducted on tumor-bearing mice revealed that mice treated with SCP nanoparticles exhibited a survival rate of 40 %, which was significantly higher than the 0 % and 20 % survival rates observed in the control and SCP-treated groups, respectively. These findings suggest that SCP nanoparticles, possessing antioxidative properties and controllable sizes, hold potential for precision nutrition in the field of cancer treatment.
肝癌是一种全球发病率不断上升的恶性肿瘤，在实现有效治疗效果方面构成了重大挑战。海参肽（SCP）作为一种源自食物的营养物质，已显示出有希望的抗癌作用。因此，我们探索了纳米递药系统来包裹 SCP，以增强其在胃肠道中的稳定性并改善在肿瘤微环境中的吸收。本研究旨在通过一锅曼尼希缩合方法，使用 SCP、原花青素（PCs）和香草醛通过分子组装来开发尺寸可控的多功能纳米粒子。这些食品级纳米粒子具有水溶性，并表现出球形结构，其尺寸范围为 441 至 1360nm，具体取决于反应物的浓度。体外细胞实验表明，用 PCs 修饰的 SCP 纳米粒子可有效减少 HO 和丙烯酰胺产生的活性氧，同时保持正常的线粒体膜电位。此外，对荷瘤小鼠进行的体内营养干预研究表明，用 SCP 纳米粒子治疗的小鼠的存活率为 40%，明显高于对照组和 SCP 处理组的 0%和 20%的存活率。这些发现表明，具有抗氧化特性和可控尺寸的 SCP 纳米粒子在癌症治疗领域的精准营养方面具有潜力。
