School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan1, Ganjingzi District, Dalian 116034, Liaoning, China.
National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
J Agric Food Chem. 2021 Feb 10;69(5):1466-1477. doi: 10.1021/acs.jafc.0c04895. Epub 2021 Jan 28.
The purpose of this study was to construct a delivery system using a microfluidic chip to protect procyanidins (PCs) and to achieve their pH-controlled release in simulated gastrointestinal fluid. The microfluidic chip was designed and fabricated to generate water-in-water-in-oil (W/W/O) templates for the preparation of sodium alginate/chitosan microparticles with a uniform size and core-shell structure, using an internal-external gelation method. Compared with free PCs, the stability of PCs embedded in microparticles was improved and a pH stimulus-responsive release of PCs from microparticles was observed under neutral pH conditions. The delivery system of microparticles was nontoxic and showed an inhibitory effect on the decrease of mitochondrial membrane potential in Caco-2 cells caused by HO and acrylamide. This work provided a method for fabricating compact microfluidic chips to prepare a pH stimulus-responsive PCs delivery system with improved stability, which may have potential applications in the delivery of other nutrients.
本研究旨在构建一种使用微流控芯片的递送系统，以保护原花青素（PCs），并在模拟胃肠道液中实现其 pH 控制释放。该微流控芯片经过设计和制造，可生成水包水包油（W/W/O）模板，用于采用内外凝胶化法制备具有均匀大小和核壳结构的海藻酸钠/壳聚糖微球。与游离 PCs 相比，嵌入微球中的 PCs 的稳定性得到提高，并且在中性 pH 条件下观察到 PCs 从微球中响应 pH 刺激的释放。微球的递送系统是无毒的，并表现出对 HO 和丙烯酰胺引起的 Caco-2 细胞线粒体膜电位降低的抑制作用。这项工作提供了一种制造紧凑型微流控芯片的方法，用于制备具有提高的稳定性的 pH 刺激响应 PCs 递送系统，该系统可能在其他营养物质的递送中具有潜在应用。
