Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
Food Funct. 2022 Apr 4;13(7):4069-4085. doi: 10.1039/d1fo04227k.
This study aimed to assess the influence of dietary supplementation of ε-polylysine on the gut microbiota and host nutrient metabolism, which is not systematically discussed by multi-omics analysis. A total of 40 mice were randomly divided into two groups exposed to either a basal diet (AIN-76A) or a basal diet with 150 ppm ε-polylysine. Fecal samples were collected for gut bacteria identification. Liver and plasma samples were collected for metabolomic and proteomic analyses. The results showed that ε-polylysine decreased the body weight of mice and affected the presence of certain types of intestinal microorganisms. The richness of the microbiota and number of phyla increased with age. ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins. The metabolite profiling showed that lipid and lipid-like molecules metabolites occupied the majority percent of plasma and liver metabolites. Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism. In conclusion, dietary ε-polylysine improved the immunity of growing mice, and had a greater effect on the anabolism of nutrients in adult mice.
本研究旨在评估ε-聚赖氨酸对肠道微生物群和宿主营养代谢的影响，这一点尚未通过多组学分析进行系统讨论。将 40 只小鼠随机分为两组，分别暴露于基础饮食（AIN-76A）或基础饮食加 150ppmε-聚赖氨酸。收集粪便样本进行肠道细菌鉴定。收集肝脏和血浆样本进行代谢组学和蛋白质组学分析。结果表明，ε-聚赖氨酸降低了小鼠的体重，并影响了某些类型的肠道微生物的存在。微生物丰富度和菌门数量随年龄增长而增加。ε-聚赖氨酸影响属和种的存在，或者调节或者参与能量、氮、氨基酸、脂质、碳水化合物、聚糖、辅因子和维生素的代谢。代谢物分析表明，脂质和类脂质代谢物在血浆和肝脏代谢物中占据大多数。此外，ε-聚赖氨酸调节代谢途径中关键代谢物及其相关代谢酶的作用，特别是磷脂代谢。总之，膳食ε-聚赖氨酸提高了生长中的小鼠的免疫力，对成年小鼠的营养物质合成代谢有更大的影响。
