Huang Weichen, Guo Yanshan, Ge Chaoyue, Lv Yujie, Zhan Shenao, Shen Xinyu, Hu Zhaoying, Wu Lianchi, Luo Xinyu, Yu Dongyou, Liu Bing
Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China.
Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China; Hainan Institute, Zhejiang University, Sanya 572000, PR China.
Poult Sci. 2025 Feb;104(2):104774. doi: 10.1016/j.psj.2025.104774. Epub 2025 Jan 3.
This study was conducted to investigate the effects of dietary ε-polylysine supplementation on growth performance, antioxidant capacity, intestinal morphology, and gut microbiota in broilers fed a lysine-deficient diet. A total of 960 one-day-old yellow-feathered broilers were randomly allocated into six groups with eight replicates and 20 birds per replicate. Birds in the six groups were fed a lysine-deficient basal diet (-0.20 % lysine, NC) or basal diets supplemented with 0.20 % L-lysine hydrochloride (normal lysine level, PC) or varying levels (0.10 %, 0.20 %, 0.30 %, or 0.40 %) of ε-polylysine hydrochloride for 10 weeks. Results showed that compared with the NC group, dietary supplementation with 0.10 % and 0.20 % of ε-polylysine improved growth performance, meat quality and intestinal morphology, evidenced by increased average daily gain, decreased feed-to-gain ratio, increased water holding capacity of breast muscle, and increased jejunal and ileal villus height in ε-polylysine-treated broilers (P < 0.05). In addition, 0.10 % and 0.20 % of ε-polylysine supplementation improved serum nitrogen metabolism, immune and antioxidant capacity to varying degrees. No significant diffrences in growth performance, meat quality, or intestinal morphology were observed among PC and ε-polylysine-supplemented groups (P > 0.05). Interestingly, compared with the PC group, dietary 0.10 % of ε-polylysine positively altered the microbiota composition of broilers, evidenced by the increased the relative abundance of beneficial bacteria (e.g., Faecalibacterium and Phascolarctobacterium) and decreased the abundance of harmful bacteria (e.g., Lachnoclostridium and Peptococcus). In conclusion, diets supplemented with ε-polylysine have beneficial effects on growth performance, meat quality, and gut health of broilers via replenishing lysine or favorably altering immune and antioxidant capacity and gut microbiota. The recommended supplemental amount of ε-polylysine in lysine-deficient diets (L-lysine content in the starter and growing diet is 0.95 % and 0.80 % of the basal diet, respectively) is 0.10 %-0.20 %.
本研究旨在探讨在饲喂低赖氨酸日粮的肉鸡中添加膳食ε-聚赖氨酸对其生长性能、抗氧化能力、肠道形态和肠道微生物群的影响。总共960只1日龄黄羽肉鸡被随机分为6组，每组8个重复，每个重复20只鸡。6组鸡分别饲喂低赖氨酸基础日粮（赖氨酸含量-0.20%，NC）或添加0.20% L-盐酸赖氨酸的基础日粮（正常赖氨酸水平，PC）或不同水平（0.10%、0.20%、0.30%或0.40%）的盐酸ε-聚赖氨酸，持续10周。结果表明，与NC组相比，添加0.10%和0.20% ε-聚赖氨酸可改善生长性能、肉质和肠道形态，表现为ε-聚赖氨酸处理的肉鸡平均日增重增加、料重比降低、胸肌持水能力提高以及空肠和回肠绒毛高度增加（P < 0.05）。此外，添加0.10%和0.20% ε-聚赖氨酸可不同程度地改善血清氮代谢、免疫和抗氧化能力。PC组和添加ε-聚赖氨酸的组在生长性能、肉质或肠道形态方面未观察到显著差异（P > 0.05）。有趣的是，与PC组相比，日粮中添加0.10% ε-聚赖氨酸可使肉鸡的微生物群组成发生积极变化，表现为有益菌（如粪杆菌属和考拉杆菌属）的相对丰度增加，有害菌（如厌氧消化链球菌和消化球菌属）的丰度降低。总之，添加ε-聚赖氨酸的日粮通过补充赖氨酸或有利地改变免疫和抗氧化能力以及肠道微生物群，对肉鸡的生长性能、肉质和肠道健康具有有益影响。在低赖氨酸日粮（起始料和生长料中L-赖氨酸含量分别为基础日粮的0.95%和0.80%）中，ε-聚赖氨酸的推荐添加量为0.10%-0.20%。
