Endo Yoshinori, Kamei Ken-Ichiro, Hasegawa Kouichi, Okita Keisuke, Ito Hideyuki, Terada Shiho, Inoue-Murayama Miho
Wildlife Research Centre, Kyoto University, Kyoto, Japan.
Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan.
Stem Cells Dev. 2022 May;31(9-10):250-257. doi: 10.1089/scd.2021.0253. Epub 2022 Apr 19.
Induced pluripotent stem cells (iPSCs) can serve as a biological resource for functional and conservation research for various species. This realization has led to the generation of iPSCs from many species, including those identified as endangered. However, the understanding of species variation in mammalian iPSCs remains largely unknown. To gain insight into species variation in iPSCs, we generated iPSCs from a new species Grevy's zebra (; gz-iPSCs), which has been listed as endangered in the IUCN (International Union for Conservation of Nature) Red List. We isolated primary fibroblast cells from an individual and successfully reprogrammed them into iPSCs. The generated gz-iPSCs continued to grow under primed-type culture condition and showed pluripotency and differentiation potential. To describe the molecular characteristics of gz-iPSCs, we performed RNA sequencing analysis. The gz-iPSC transcriptome showed robust expression of pluripotency-associated genes reported in human and mouse, suggesting evolutionary conservation among the species. This study provides insight into the iPSCs from a rare species and helps the understanding of the gene expression basis underlying mammalian pluripotent stem cells.
诱导多能干细胞（iPSC）可作为各种物种功能和保护研究的生物资源。这一认识促使人们从包括濒危物种在内的许多物种中生成了iPSC。然而，对哺乳动物iPSC中物种差异的了解仍然知之甚少。为了深入了解iPSC中的物种差异，我们从一个新物种细纹斑马（gz-iPSC）中生成了iPSC，该物种已被列入国际自然保护联盟（IUCN）红色名录中的濒危物种。我们从一只个体中分离出原代成纤维细胞，并成功地将它们重编程为iPSC。生成的gz-iPSC在原始型培养条件下持续生长，并表现出多能性和分化潜能。为了描述gz-iPSC的分子特征，我们进行了RNA测序分析。gz-iPSC转录组显示出在人类和小鼠中报道的多能性相关基因的强烈表达，表明这些物种之间存在进化保守性。这项研究为来自稀有物种的iPSC提供了见解，并有助于理解哺乳动物多能干细胞的基因表达基础。
