Department of Medicine, University of Verona, 37100, Verona, Italy.
Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37100, Verona, Italy.
Stem Cell Rev Rep. 2022 Jun;18(5):1865-1874. doi: 10.1007/s12015-022-10361-9. Epub 2022 Mar 22.
Stem cells functions are regulated by different factors and non-conding RNAs, such as microRNA. MiRNAsplay an important role in modulating the expression of genes involved in the commitment and differentiation of progenitor cells. MiRNAs are post transcriptional regulators which may be modulated by physical exercise. MiRNAs, by regulating different signaling pathways, play an important role in myogenesis as well as in muscle activity. MiRNAs quantification may be considered for evaluating physical performance or muscle recovery. With the aim to identify specific miRNAs potentially involved in myogenesis and modulated by physical activity, we investigated miRNAs expression following physical performance in Peripheral Blood Mononuclear Cells (PBMCs) and in sera of half marathon (HM) runnners. The effect of runners sera on Myogenesis in in vitro cellular models was also explored. Therefore, we performed Microarray Analysis and Real Time PCR assays, as well as in vitro cell cultures analysis to investigate myogenic differentiation. Our data demonstrated gender-specific expression patterns of PBMC miRNAs before physical performance. In particular, miR223-3p, miR26b-5p, miR150-5p and miR15-5p expression was higher, while miR7a-5p and miR7i-5p expression was lower in females compared to males. After HM, miR152-3p, miR143-3p, miR27a-3p levels increased while miR30b-3p decreased in both females and males: circulating miRNAs mirrored these modulations. Furthermore, we also observed that the addition of post-HM participants sera to cell cultures exerted a positive effect in stimulating myogenesis. In conclusion, our data suggest that physical activity induces the modulation of myogenesis-associated miRNAs in bothfemales and males, despite the gender-associated different expression of certain miRNAs, Noteworthy, these findings might be useful for evaluating potential targets for microRNA based-therapies in diseases affecting the myogenic stem cells population.
干细胞的功能受不同因素和非编码 RNA 的调节，如 microRNA。miRNA 在调节参与祖细胞定向和分化的基因表达方面发挥重要作用。miRNA 是转录后调节因子，可能受到体育锻炼的调节。miRNA 通过调节不同的信号通路，在肌发生以及肌肉活动中发挥重要作用。miRNA 的定量分析可用于评估身体表现或肌肉恢复。为了鉴定潜在参与肌发生并受体育活动调节的特定 miRNA，我们研究了外周血单核细胞（PBMC）和半程马拉松（HM）跑步者血清中体力活动后的 miRNA 表达。还探讨了跑步者血清对体外细胞模型中肌发生的影响。因此，我们进行了微阵列分析和实时 PCR 检测，以及体外细胞培养分析，以研究肌发生分化。我们的数据表明，体力活动前 PBMC miRNA 存在性别特异性表达模式。具体而言，与男性相比，女性 miR223-3p、miR26b-5p、miR150-5p 和 miR15-5p 的表达更高，而 miR7a-5p 和 miR7i-5p 的表达更低。在 HM 之后，miR152-3p、miR143-3p、miR27a-3p 的水平在女性和男性中均增加，而 miR30b-3p 降低：循环 miRNA 反映了这些调节。此外，我们还观察到添加 HM 后参与者的血清对细胞培养具有刺激肌发生的积极作用。总之，我们的数据表明，体育活动诱导了女性和男性中与肌发生相关的 miRNA 的调节，尽管某些 miRNA 的表达存在性别相关的差异。值得注意的是，这些发现可能有助于评估影响肌源性干细胞群体的疾病的基于 miRNA 的治疗的潜在靶点。
