Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, People's Republic of China.
Shenzhen Bay Laboratory, Shenzhen, Guangdong 518132, People's Republic of China.
J Neural Eng. 2022 Apr 4;19(2). doi: 10.1088/1741-2552/ac5fcc.
. Glioma growth may cause pervasive disruptions of brain vascular structure and function. Revealing both structural and functional alterations at a fine spatial scale is challenging for existing imaging techniques, which could confound the understanding of the basic mechanisms of brain diseases.. In this study, we apply photoacoustic microscopy with a high spatial-temporal resolution and a wide field of view to investigate the glioma-induced alterations of cortical vascular morphology, hemodynamic response, as well as functional connectivity at resting- and stimulated- states.We find that glioma promotes the growth of microvessels and leads to the increase of vascular proportion in the cerebral cortex by deriving structural parameters. The glioma also causes the loss of response in the ipsilateral hemisphere and abnormal response in the contralateral hemisphere, and further induces brain-wide alterations of functional connectivity in resting and stimulated states.The observed results show the foundation of employing photoacoustic microscopy as a potential technique in revealing the underlying mechanisms of brain diseases.
. 胶质瘤的生长可能导致大脑血管结构和功能的普遍紊乱。现有的成像技术在精细的空间尺度上揭示结构和功能的改变具有挑战性，这可能会混淆对脑部疾病基本机制的理解。在这项研究中，我们应用具有高时空分辨率和宽视场的光声显微镜来研究胶质瘤诱导的皮质血管形态、血流动力学反应以及静息和刺激状态下的功能连接的改变。我们发现，胶质瘤通过推导结构参数促进微血管生长，并导致大脑皮层中血管比例增加。胶质瘤还导致同侧半球反应丧失和对侧半球异常反应，并进一步诱导静息和刺激状态下全脑功能连接的改变。观察到的结果表明，光声显微镜作为一种揭示脑部疾病潜在机制的潜在技术具有应用基础。
