Pourmirzaei Fatemeh, Ranjbaran Mina, Kadkhodaee Mehri, Kianian Farzaneh, Lorian Keivan, Abdi Arash, Hajiaghaei Mahdi, Seifi Behjat
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Reproductive Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Iran J Basic Med Sci. 2021 Nov;24(11):1554-1560. doi: 10.22038/IJBMS.2021.59375.13183.
One of the problems caused by infectious diseases is the decrease in sperm count and motility. Tannic acid is known as an anti-oxidant and anti-inflammatory agent. In this study, Cecal Ligation and Puncture (CLP) sepsis model was induced to investigate the effect of tannic acid on oxidative stress and inflammation in testicular and sperm structure and function.
Twenty-four male Wistar rats (250-300 g) were randomly divided into 3 groups of 8: 1) sham, 2) sepsis, and 3) sepsis + tannic acid (20 mg/kg at 6, 12, and 24 hr after sepsis induction). Thirty hours after induction of sepsis, testicular samples were collected to measure SOD activity and MDA, IL-6, and TNF-α levels. Another part of the testis was fixed in 10% formalin for histological examinations.
In the sepsis group, testicular MDA, TNF-α, and IL-6 levels increased and SOD activity decreased compared with the sham group. In addition, the percentage of motile sperm and the survival rate of sperm decreased significantly in the sepsis group. Administration of tannic acid significantly decreased inflammatory markers (TNF-α and IL-6) and MDA levels and increased SOD activity. Furthermore tannic acid significantly improved sperm parameters and increased sperm and animal survival rates.
The results of this study showed that the reproductive system may be strongly affected by the conditions created during sepsis. Tannic acid improved reproductive dysfunction in sepsis by reducing oxidative stress and inflammation.
传染病引发的问题之一是精子数量和活力下降。单宁酸是一种已知的抗氧化剂和抗炎剂。在本研究中，通过诱导盲肠结扎和穿刺（CLP）脓毒症模型来研究单宁酸对睾丸氧化应激、炎症以及精子结构和功能的影响。
将24只雄性Wistar大鼠（250 - 300克）随机分为3组，每组8只：1）假手术组，2）脓毒症组，3）脓毒症 + 单宁酸组（脓毒症诱导后6、12和24小时给予20毫克/千克单宁酸）。脓毒症诱导30小时后，采集睾丸样本以测量超氧化物歧化酶（SOD）活性以及丙二醛（MDA）、白细胞介素 - 6（IL - 6）和肿瘤坏死因子 - α（TNF - α）水平。睾丸的另一部分固定在10%福尔马林中用于组织学检查。
与假手术组相比，脓毒症组睾丸MDA、TNF - α和IL - 6水平升高，SOD活性降低。此外，脓毒症组活动精子百分比和精子存活率显著降低。给予单宁酸可显著降低炎症标志物（TNF - α和IL - 6）和MDA水平，并提高SOD活性。此外，单宁酸显著改善精子参数并提高精子和动物存活率。
本研究结果表明，脓毒症期间产生的状况可能会对生殖系统产生强烈影响。单宁酸通过减轻氧化应激和炎症改善脓毒症中的生殖功能障碍。
