使用酶联免疫吸附测定法测量免疫胶固素。
Use of ELISA for measuring immunoconglutinins.
作者信息
Hautanen A, Koistinen V, Penttinen K, Wager O
出版信息
J Immunol Methods. 1978;21(3-4):335-40. doi: 10.1016/0022-1759(78)90160-6.
An enzyme-linked immunosorbent assay (ELISA) for detection of immunoconglutinins (IKs) has been developed. IKs are allowed to bind to solid-phase C3 and are then assayed using alkaline phosphatase coupled to anti-IgG or anti-IgM. IK activity was detected in some patients' sera up to 50,000-fold dilution. Significant correlation (r = 0.65) was obtained between the levels of IgM-IKs measured by ELISA and by conventional hemagglutination assay, IgG-IKs and hemagglutination titers did not correlate (r = -0.15).
一种用于检测免疫粘连素（IKs）的酶联免疫吸附测定（ELISA）已被开发出来。使IKs与固相C3结合，然后使用与抗IgG或抗IgM偶联的碱性磷酸酶进行检测。在一些患者血清中，稀释至50000倍时仍能检测到IK活性。通过ELISA测量的IgM-IKs水平与传统血凝试验测量的水平之间存在显著相关性（r = 0.65），而IgG-IKs与血凝滴度之间无相关性（r = -0.15）。
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