Camargo M E, Ferreira A W, Mineo J R, Takiguti C K, Nakahara O S
Infect Immun. 1978 Jul;21(1):55-8. doi: 10.1128/iai.21.1.55-58.1978.
Enzyme-linked immunosorbent assay (ELISA) for toxoplasmosis was evaluated in serum samples presenting defined toxoplasmosis serological patterns, as determined by results in immunoglobulin (Ig)G and IgM immunofluorescence (IgG-IF, IgM-IF), hemagglutination, and complement fixation tests. ELISA was carried out with alkaline phosphatase-labeled anti-IgG and anti-IgM antibodies. Serum titer was expressed as the serum dilution end point determined by mere observation of color development in the test. A straight agreement was found between IgG-ELISA and IgG-IF titers, both in group A sera of ancient infections (patterns II and III) and group B sera of recent infections (pattern I). A similar agreement was found between IgG-ELISA and hemagglutination titers in group A sera, for which coincident IgG-IF and hemagglutination titers are also frequent. However, in group B sera, in spite of the same toxoplasma extract being used to sensitize both plastic surfaces and erythrocytes, IgG-ELISA titers were much higher than hemagglutination titers, in a way similar to that observed for IgG-IF titers. IgM-ELISA was positive in every group B serum, with higher titers than corresponding IgM-IF titers. Occasional low-titered positive IgM-ELISA results were seen for group A sera, sometimes due to IgM-antiglobulin antibodies. An easy test to perform, ELISA seems to be an adequate substitute for toxoplasmosis IF tests for routine purposes.
通过免疫球蛋白（Ig）G和IgM免疫荧光法（IgG-IF、IgM-IF）、血凝试验和补体结合试验的结果，对呈现明确弓形虫病血清学模式的血清样本进行了弓形虫病酶联免疫吸附测定（ELISA）评估。使用碱性磷酸酶标记的抗IgG和抗IgM抗体进行ELISA检测。血清滴度表示为仅通过观察试验中颜色变化确定的血清稀释终点。在A组既往感染血清（模式II和III）和B组近期感染血清（模式I）中，IgG-ELISA和IgG-IF滴度之间发现了直接一致性。在A组血清中，IgG-ELISA和血凝滴度之间也发现了类似的一致性，A组血清中IgG-IF和血凝滴度也经常一致。然而，在B组血清中，尽管使用相同的弓形虫提取物对塑料表面和红细胞进行致敏，但IgG-ELISA滴度远高于血凝滴度，这与IgG-IF滴度的情况类似。IgM-ELISA在每组B血清中均为阳性，滴度高于相应的IgM-IF滴度。A组血清偶尔会出现低滴度阳性IgM-ELISA结果，有时是由于IgM抗球蛋白抗体。ELISA操作简便，似乎可以作为常规用途的弓形虫病IF试验的适当替代方法。
