Federated Department of Biological Sciences, New Jersey Institute of Technology and Rutgers University, Newark, NJ, USA.
Methods Mol Biol. 2022;2468:319-328. doi: 10.1007/978-1-0716-2181-3_17.
Laser microsurgery allows the user to ablate cell bodies or disconnect nerve fibers by using a laser microbeam focused through a microscope. This technique was pioneered in C. elegans where it led to exciting discoveries in the fields of development and neurobiology. All neurons studied so far in C. elegans can regenerate and regrow axons and dendrites after injury, allowing studies of the molecular and cellular basis of neuroregeneration. In this chapter, we describe how to assemble and operate a platform for Yb-doped fiber laser microsurgery. The novel laser setup described here is a more robust, lower cost, and user-friendly alternative to other femtosecond-pulsed laser systems.
激光显微手术允许用户通过聚焦显微镜的激光微束来消融细胞体或断开神经纤维。这项技术在秀丽隐杆线虫中得到了先驱性的应用，在那里它为发育和神经生物学领域带来了令人兴奋的发现。迄今为止，在秀丽隐杆线虫中研究的所有神经元在受伤后都可以再生和重新生长轴突和树突，这使得对神经再生的分子和细胞基础的研究成为可能。在这一章中，我们描述了如何组装和操作掺镱光纤激光显微手术平台。与其他飞秒脉冲激光系统相比，这里描述的新型激光装置具有更高的稳定性、更低的成本和更好的用户友好性。
