Pokharel Kisun, Peippo Jaana, Weldenegodguad Melak, Honkatukia Mervi, Li Meng-Hua, Kantanen Juha
Natural Resources, Natural Resources Institute Finland (Luke), 31600 Jokioinen, Finland.
Production Systems, Natural Resources Institute Finland (Luke), 31600 Jokioinen, Finland.
Genes (Basel). 2020 Apr 10;11(4):415. doi: 10.3390/genes11040415.
The majority of pregnancy loss in ruminants occurs during the preimplantation stage, which is thus the most critical period determining reproductive success. Here, we performed a comparative transcriptome study by sequencing total mRNA from (CL) collected during the preimplantation stage of pregnancy in Finnsheep, Texel and F1 crosses. A total of 21,287 genes were expressed in our data. Highly expressed autosomal genes in the CL were associated with biological processes such as progesterone formation (, , and ) and embryo implantation (e.g., , and ). Among the list of differentially expressed genes, sialic acid-binding immunoglobulin (Ig)-like lectins (, , ), ribosomal proteins (, ) and chemokines (, CCL24, , ) were upregulated in Finnsheep, while four multidrug resistance-associated proteins (MRPs) were upregulated in Texel ewes. A total of 17 known genes and two uncharacterized non-coding RNAs (ncRNAs) were differentially expressed in breed-wise comparisons owing to the flushing diet effect. The significantly upregulated gene indicated potential for embryonic diapause in Finnsheep and F1. Moreover, we report, for the first time in any species, several genes that are active in the CL during early pregnancy (including , , , and ).
反刍动物的大多数妊娠丢失发生在植入前阶段，因此该阶段是决定繁殖成功的最关键时期。在此，我们通过对芬兰羊、特克塞尔羊及其F1杂交种妊娠植入前阶段收集的黄体（CL）中的总mRNA进行测序，开展了一项比较转录组研究。我们的数据中共表达了21287个基因。CL中高表达的常染色体基因与孕酮生成（如、和）以及胚胎着床（如、和）等生物学过程相关。在差异表达基因列表中，唾液酸结合免疫球蛋白（Ig）样凝集素（、、）、核糖体蛋白（、）和趋化因子（、CCL24、、）在芬兰羊中上调，而四种多药耐药相关蛋白（MRP）在特克塞尔母羊中上调。由于催情补饲效应，共有17个已知基因和两个未鉴定的非编码RNA（ncRNA）在品种间比较中差异表达。显著上调的基因表明芬兰羊和F1中存在胚胎滞育的可能性。此外，我们首次在任何物种中报道了几个在妊娠早期CL中活跃的基因（包括、、、和）。
