Deschepper C F, Mellon S H, Cumin F, Baxter J D, Ganong W F
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7552-6. doi: 10.1073/pnas.83.19.7552.
Renin gene expression in cells and tissues of the rat was examined by in situ hybridization histochemistry and immunocytochemistry. By using a mouse cDNA probe, hybridization histochemistry revealed renin mRNA in the renal juxtaglomerular cells, testicular Leydig cells, adrenal zona glomerulosa cells, the intermediate lobe of the pituitary, and scattered cells of the anterior lobe of the pituitary. With four separate antisera to mouse submaxillary renin, there was immunoreactivity in the renal juxtaglomerular cells. However, only one of the antisera stained the Leydig cells, a second stained the adrenal zona glomerulosa, a third stained the intermediate lobe of the pituitary, and a fourth stained scattered cells of the anterior lobe of the pituitary that were identified as gonadotrophs. The variations with the different antisera in detecting extrarenal renin are unexplained but could imply that posttranslational proteolysis or glycosylation of preprorenin varies in different tissues with consequent variations in immunoreactivity. The finding of renin mRNA and renin-like immunoreactivity in these tissues supports the notion that these tissues are sites for production of renin.
通过原位杂交组织化学和免疫细胞化学方法检测了大鼠细胞和组织中的肾素基因表达。使用小鼠cDNA探针，杂交组织化学显示在肾球旁细胞、睾丸间质细胞、肾上腺球状带细胞、垂体中间叶以及垂体前叶的散在细胞中有肾素mRNA。用四种针对小鼠颌下腺肾素的不同抗血清检测，肾球旁细胞有免疫反应性。然而，只有一种抗血清能使间质细胞染色，第二种能使肾上腺球状带染色，第三种能使垂体中间叶染色，第四种能使垂体前叶中被鉴定为促性腺激素细胞的散在细胞染色。不同抗血清在检测肾外肾素时出现的差异尚无法解释，但这可能意味着不同组织中前肾素原的翻译后蛋白水解或糖基化存在差异，并导致免疫反应性的变化。在这些组织中发现肾素mRNA和肾素样免疫反应性支持了这些组织是肾素产生部位的观点。
