Mirsalehi Marjan, Malihi Golrokh, Bahrami Eshagh, Akbarnejad Zeinab, Ahmadi Sayedali
ENT and Head & Neck Research Center and Department, Hazrat Rasoul Hospital, The Five Senses Health institute, Iran University of Medical Sciences, Tehran, Iran.
Fargol Research Group, Ballwin, Missouri, USA.
Med J Islam Repub Iran. 2021 Oct 20;35:138. doi: 10.47176/mjiri.35.138. eCollection 2021.
Lamotrigine (LTG) is an antiepileptic drug used in the treatment of seizures, mood disorders, and cognitive problems. The cardiac effects of LTG, such as LTG toxicity and SUEDP, have been studied. This is an in vitro study examining the effect of LTG on isolated atria of guinea pigs. The atria of 21 male Guinea pigs were isolated and stabilized in Krebs-solution and physiologic condition. The rhythm of contraction, contractile force, and heart rate were recorded. In 7 atria, LTG at the doses of 2, 4, 8, and 16 mg/mL were added and the contractile forces and heart rates were recorded and compared together. In the next step, in 14 atria, 8 were pretreated with LTG, and 6 without pretreatment were exposed to ouabain, and the times of the onset of effect, arrhythmia, and asystole were recorded. The statistical comparisons were made by using Student's t test and repeated measure analysis of variance followed by the Bonferroni method. Lamotrigine (4, 8, and 16 mg/mL) significantly decreased the heart rate and contractile force of the isolated guinea pigs' atria (P <.001). Pretreatment with LTG significantly increased the mean time of onset of the effect of ouabain, the onset of ouabain-induced arrhythmia, and time of ouabain-induced asystole (P <.001). LTG reduces the heart rate and contractile force, and also inhibit ouabain induced-arrhythmia of the isolated atria of guinea pigs.
拉莫三嗪（LTG）是一种用于治疗癫痫发作、情绪障碍和认知问题的抗癫痫药物。已经对LTG的心脏效应，如LTG毒性和SUEDP进行了研究。这是一项体外研究，旨在检验LTG对豚鼠离体心房的作用。将21只雄性豚鼠的心房分离出来，在 Krebs 溶液和生理条件下使其稳定。记录收缩节律、收缩力和心率。在7个心房中，加入2、4、8和16mg/mL剂量的LTG，并记录和比较收缩力和心率。下一步，在14个心房中，8个用LTG预处理，6个未预处理的暴露于哇巴因，记录起效时间、心律失常和心搏停止的时间。采用 Student's t 检验和重复测量方差分析，随后采用 Bonferroni 方法进行统计比较。拉莫三嗪（4、8和16mg/mL）显著降低了豚鼠离体心房的心率和收缩力（P<.001）。用LTG预处理显著增加了哇巴因作用的平均起效时间、哇巴因诱导的心律失常的起效时间和哇巴因诱导的心搏停止的时间（P<.001）。LTG降低心率和收缩力，并抑制哇巴因诱导的豚鼠离体心房心律失常。
