Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, People's Republic of China.
Aesthetic Plast Surg. 2022 Oct;46(5):2517-2525. doi: 10.1007/s00266-022-02857-z. Epub 2022 Mar 24.
Adipose browning occurs after white fat transfer. But its location and effects on fat graft survival remains controversial. This study was performed to locate the browning of fat grafts, and to explore the effects of quercetin on fat graft browning and fat graft survival.
Human fat granules were injected into the subcutaneous layer of 12 nude mice. Control group was injected with fat granules and 10% of normal saline, while quercetin group was injected with fat granules and 10% of quercetin. The graft samples (n = 6 for each group) were obtained in weeks 2, 4, 8 and 12. Weight retention rate of the grafts was calculated. Gene and protein expression of mitochondrial markers (silent information regulator 1, SIRT1; heat shock protein 60, HSP60), browning marker (uncoupling protein 1, UCP1), peroxisome proliferator-activated receptor-γ (PPAR-γ), vascular endothelial growth factor A (VEGF-A) were evaluated. Hematoxylin and eosin staining and anti-UCP1 staining were performed.
Clusters of small multilocular beige adipocytes were observed in the periphery of fat grafts. Compared with control group, quercetin group had a higher weight retention rate, a higher gene/protein expression of SIRT1, HSP60, UCP1, PPAR-γ and VEGF-A, and a higher occurrence of peripheral adipose browning.
Peripherally located adipose browning occurred after white fat transfer. It can be enhanced by the addition of quercetin through promoting mitochondrial function of fat cells, and may be one of the mechanisms that quercetin improves fat graft survival.
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脂肪褐变发生在白色脂肪转移之后。但是其位置和对脂肪移植物存活的影响仍存在争议。本研究旨在定位脂肪移植物的褐变，并探讨槲皮素对脂肪移植物褐变和脂肪移植物存活的影响。
将人脂肪颗粒注入 12 只裸鼠的皮下层。对照组注射脂肪颗粒和 10%生理盐水，槲皮素组注射脂肪颗粒和 10%槲皮素。在第 2、4、8 和 12 周时获取移植物样本（每组 6 个样本）。计算移植物的重量保留率。评估线粒体标志物（沉默信息调节因子 1、SIRT1；热休克蛋白 60、HSP60）、褐变标志物（解偶联蛋白 1、UCP1）、过氧化物酶体增殖物激活受体-γ（PPAR-γ）、血管内皮生长因子 A（VEGF-A）的基因和蛋白表达。进行苏木精和伊红染色和抗 UCP1 染色。
在脂肪移植物的外周观察到小多房米色脂肪细胞簇。与对照组相比，槲皮素组的重量保留率更高，SIRT1、HSP60、UCP1、PPAR-γ 和 VEGF-A 的基因/蛋白表达更高，外周脂肪褐变的发生率更高。
白色脂肪转移后会发生外周脂肪褐变。通过促进脂肪细胞的线粒体功能，槲皮素可以增强这种褐变，这可能是槲皮素改善脂肪移植物存活的机制之一。
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