Zhang Tianjia, Dai Jiewen, Xu Yuting, Yu Liya, Wang Xudong
Shanghai Ninth People's Hospital, Department of Oral and Craniomaxillofacial Surgery, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, 639 Meiji Road, Huangpu District, Shanghai, 200011, China.
Aesthetic Plast Surg. 2021 Oct;45(5):2417-2422. doi: 10.1007/s00266-021-02336-x. Epub 2021 Jun 1.
The present study aimed to explore the efficacy and safety profile of liquid phase concentrated growth factor (LPCGF) in promoting autologous fat graft survival.
LPCGF/PRP was mixed with human fat tissues at different proportions and transplanted into nude mice. Three months after transplantation, the implanted fat tissues were retrieved for analysis. H&E staining was used to quantify the neovascularization. Immunohistochemical staining was applied to quantify the CD34-positive stem cells and the fluorescence intensity of VEGF and TGF-β.
Addition of LPCGF to autologous fat reduced the fat absorption by 5-15%, especially at the early stage, and no complications were observed. In addition, the effect was improved with increased CGF. Liquid phase concentrated growth factor improves autologous fat graft survival, and the most suitable ratio of LPCGF/fat is 1:8.
LPCGF is rich in VEGF, TGF-β and CD34-positive stem cells, which can improve the fat transplantation effect, but the specific influence of a single component requires future evaluation.
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本研究旨在探讨液相浓缩生长因子（LPCGF）在促进自体脂肪移植存活方面的疗效和安全性。
将LPCGF/PRP与人类脂肪组织按不同比例混合后移植到裸鼠体内。移植3个月后，取出植入的脂肪组织进行分析。采用苏木精-伊红（H&E）染色定量新生血管形成。应用免疫组织化学染色定量CD34阳性干细胞以及血管内皮生长因子（VEGF）和转化生长因子-β（TGF-β）的荧光强度。
向自体脂肪中添加LPCGF可使脂肪吸收率降低5%-15%，尤其是在早期，且未观察到并发症。此外，随着CGF增加，效果得到改善。液相浓缩生长因子可提高自体脂肪移植的存活率，LPCGF/脂肪的最适比例为1:8。
LPCGF富含VEGF、TGF-β和CD34阳性干细胞，可改善脂肪移植效果，但单一成分的具体影响有待未来评估。
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