Sushmitha Dindukurthi Harinathbabu, Ramesh Channaiah, Sowmya Belladamadagu Appajappa, Sushma Bai Byrojai Rao, Rawal Pinkey, Shabana Shafi Ulla Khan
Department of Pharmacology, East West College of Pharmacy, Bangalore, India.
Department of Pharmaceutical Chemistry, East West College of Pharmacy, Bangalore, India.
J Complement Integr Med. 2022 Mar 24;19(4):975-985. doi: 10.1515/jcim-2021-0515. eCollection 2022 Dec 1.
The present study was performed to evaluate the protective effects of methanol extract of against cisplatin induced.
Myelosuppression in albino Wistar rats. All experimental animals were administered with cisplatin on 1st, 3rd, 5th and 7th day to induce bone marrow toxicity and rats were treated with methanol extract of for 21 days. Blood samples were collected from all the animals on day 1st, 7th, 14th and 21st after 1 h before the administration of the drugs and hematological parameters like RBC, WBC, platelets, hemoglobin, hematocrit, eosinophils, basophils, neutrophils, lymphocytes, bleeding time and clotting time were determined were determined. At 21st, all rats were sacrificed and bone marrow samples were collected. The part of bone marrow samples was used for the determination of antioxidant enzymes and remaining were subjected to histopathological examination.
The animals of therapeutic groups administered with extract of have exhibited significant rise in hematological parameters and shorten bleeding time and clotting time when compare to toxic control animals on the day 14 and day 21. The histopathological examination revealed the regeneration of bone marrow cells in the extract treated animals. There was significant reduction in lipid peroxidation and increase in antioxidant enzymes was found in extract treated animals.
The methanol extract of of have shown significant protective effects against cisplatin induced myelosuppression in albino Wistar rats.
进行本研究以评估[具体提取物名称未给出]甲醇提取物对顺铂诱导的白化Wistar大鼠骨髓抑制的保护作用。
所有实验动物在第1、3、5和7天给予顺铂以诱导骨髓毒性，并给予大鼠[具体提取物名称未给出]甲醇提取物治疗21天。在给药前1小时，于第1、7、14和21天从所有动物采集血样，并测定红细胞、白细胞、血小板、血红蛋白、血细胞比容、嗜酸性粒细胞、嗜碱性粒细胞、中性粒细胞、淋巴细胞、出血时间和凝血时间等血液学参数。在第21天，处死所有大鼠并采集骨髓样本。部分骨髓样本用于测定抗氧化酶，其余样本进行组织病理学检查。
与毒性对照组动物相比，在第14天和第21天，给予[具体提取物名称未给出]提取物的治疗组动物的血液学参数显著升高，出血时间和凝血时间缩短。组织病理学检查显示提取物处理的动物骨髓细胞再生。提取物处理的动物脂质过氧化显著降低，抗氧化酶增加。
[具体提取物名称未给出]甲醇提取物对白化Wistar大鼠顺铂诱导的骨髓抑制具有显著保护作用。
