Sonnevend Ágnes, Alali Walid Q, Mahmoud Sara A, Ghazawi Akela, Bharathan Greeshma, Melegh Szilvia, Rizvi Tahir A, Pál Tibor
Department of Medical Microbiology and Immunology, Medical School, University of Pécs, 7624 Pécs, Hungary.
Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain 15551, United Arab Emirates.
Antibiotics (Basel). 2022 Feb 24;11(3):305. doi: 10.3390/antibiotics11030305.
Data on the prevalence of MCR-producing of animal origin are scarce from the Arabian Peninsula. We investigated the presence and variety of such strains from fecal specimens of poultry collected in four farms in the United Arab Emirates. Colonies from ten composite samples per farm grown on colistin-supplemented plates were PCR-screened for alleles of the gene. Thirty-nine isolates selected based on species, colony morphology, and plasmid profile were subjected to whole genome sequencing. The panel of their resistance and virulence genes, MLST and cgMLST were established. Transferability and incompatibility types of the MCR-plasmids were determined. positive strains were identified in 36 of the 40 samples. Thirty-four multi-drug resistant of 16 different sequence types, two , two and one were identified. Beyond various aminoglycoside, tetracycline, and co-trimoxazole resistance genes, seven of them also carried ESBL genes and one . Six IncHI2, 26 IncI2 and 4 IncX4 MCR-plasmids were mobilized, in case of the IncHI2 plasmids co-transferring ampicillin, chloramphenicol and tetracycline resistance. The diversity of positive strains suggest a complex local epidemiology calling for a coordinated surveillance including animals, retail meat and clinical cases.
来自阿拉伯半岛的关于动物源产MCR菌株流行情况的数据很少。我们调查了从阿拉伯联合酋长国四个农场收集的家禽粪便样本中此类菌株的存在情况和种类。在添加黏菌素的平板上培养的每个农场10个复合样本的菌落，针对该基因的等位基因进行PCR筛选。根据物种、菌落形态和质粒图谱选择的39株分离株进行全基因组测序。确定了它们的抗性和毒力基因、多位点序列分型（MLST）和核心多位点序列分型（cgMLST）。测定了MCR质粒的转移性和不相容类型。在40个样本中的36个中鉴定出阳性菌株。鉴定出34株具有16种不同序列类型的多重耐药菌株，2株 ，2株 和1株 。除了各种氨基糖苷类、四环素类和复方新诺明抗性基因外，其中7株还携带超广谱β-内酰胺酶（ESBL）基因和1株 。6个IncHI2、26个IncI2和4个IncX4 MCR质粒被转移，在IncHI2质粒的情况下，共同转移氨苄青霉素、氯霉素和四环素抗性。产MCR阳性菌株的多样性表明当地流行病学情况复杂，需要对动物、零售肉类和临床病例进行协调监测。
