Eltai Nahla O, Kelly Brianna, Al-Mana Hassan A, Ibrahim Emad B, Yassine Hadi M, Al Thani Asmaa, Al Maslmani Muna, Lammens Christine, Xavier Basil B, Malhotra-Kumar Surbhi
Biomedical Research Centre, Qatar University, Doha, Qatar.
Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.
Front Microbiol. 2020 Aug 24;11:1954. doi: 10.3389/fmicb.2020.01954. eCollection 2020.
This study was performed to investigate the genotypic causes of colistin resistance in 18 colistin-resistant ( = 13), ( = 3) and ( = 2) isolates from patients at the Hamad General Hospital, Qatar. MIC testing for colistin was performed using Phoenix (BD Biosciences, Heidelberg, Germany) and then verified with SensiTest Colistin (Liofilchem, Zona Ind. le, Italy). Strains determined to be resistant (MIC > 4-16 μg/mL) were then whole-genome sequenced (MiSeq, Illumina, Inc.). Sequences were processed and analysed using BacPipe v1.2.6, a bacterial whole genome sequencing analysis pipeline. Known chromosomal modifications were determined using CLC Genomics Workbench v.9.5.3 (CLCbio, Denmark). Two isolates (KPN-15 and KPN-19) harboured on the IncFII(K) plasmids, pqKPN-15 and pqKPN-19, and belonged to ST383 and ST716, respectively. One isolate harboured on the plasmid pEC-12. The other 15 isolates harboured known chromosomal mutations linked to colistin resistance in the PhoPQ two-component system. Also, three strains (KPN-9, KPN-10 and KPN-15) showed disruptions due to IS elements in . To our knowledge, this marks the first description of in of human origin in Qatar. Currently, more research is necessary to trace the source of and its variants in humans in this region.
本研究旨在调查卡塔尔哈马德总医院患者分离出的18株耐黏菌素的肺炎克雷伯菌（大肠埃希菌=13株、肺炎克雷伯菌=3株和奇异变形杆菌=2株）中耐黏菌素的基因型原因。使用Phoenix（德国海德堡BD生物科学公司）对黏菌素进行MIC检测，然后用SensiTest Colistin（意大利利奥菲勒姆公司Zona Ind. le）进行验证。确定为耐药的菌株（MIC>4-16μg/mL）随后进行全基因组测序（Illumina公司的MiSeq）。使用细菌全基因组测序分析流程BacPipe v1.2.6对序列进行处理和分析。使用CLC Genomics Workbench v.9.5.3（丹麦CLCbio）确定已知的染色体修饰。两株肺炎克雷伯菌分离株（KPN-15和KPN-19）在IncFII(K)质粒pqKPN-15和pqKPN-19上携带mcr-1，分别属于ST383和ST716。一株奇异变形杆菌分离株在pEC-12质粒上携带mcr-1。其他15株分离株在PhoPQ双组分系统中携带与耐黏菌素相关的已知染色体突变。此外，三株肺炎克雷伯菌菌株（KPN-9、KPN-10和KPN-15）由于IS元件导致mgrB中断。据我们所知，这是卡塔尔人类源肺炎克雷伯菌中mcr-1的首次描述。目前，有必要进行更多研究以追踪该地区人类中mcr-1及其变体的来源。
