Albishtue Abdulla A, Yimer Nurhusien, Zakaria Md Zuki A, Haron Abd Wahid, Babji Abd Salam, Abubakar Adamu A, Baiee Falah H, Almhanna Hazem Kareem, Almhanawi Bahaa H
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Department of Anatomy and Histology, Faculty of Veterinary Medicine, University of Kufa, Najaf, Iraq.
Vet World. 2019 Jul;12(7):1013-1021. doi: 10.14202/vetworld.2019.1013-1021. Epub 2019 Jul 12.
This study aimed to evaluate the protective effect of edible bird's nest (EBN) supplement on the uteri of rats exposed to lead acetate (LA) toxicity.
Five treatment groups were established as follows: Group 1 (C), which was given distilled water; Group 2 (T0), which was administered with LA (10 mg/kg body weight [BW]); and Groups 3 (T1), 4 (T2), and 5 (T3), which were given LA (10 mg/kg BW) plus graded concentrations of 30, 60, and 120 mg/kg BW of EBN, respectively. Rats were euthanized at week 5 to collect blood for superoxide dismutase (SOD) assay, and uterus for histomorphological study and expression analyses of epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and proliferating cell nuclear antigen (PCNA).
Results revealed that LA causes destruction of uterine lining cells and necrosis of uterine glands of exposed rats without EBN supplement while the degree of damage decreased among EBN treated groups; T3 showed the highest ameliorating effect against LA toxicity, as well as an increased number of uterine glands. Increased levels of SOD were also achieved in EBN supplemented groups than the controls. Results of immunohistochemistry showed significantly higher expressions of EGF, VEGF, and PCNA levels (p<0.05) in T3 compared to other treatments. EBN maintained upregulation of antioxidant - reactive oxygen species balance.
The findings showed that EBN could ameliorate the detrimental effects of LA toxicity on the uterus possibly by enhancing enzymatic antioxidant (SOD) activity as well as expressions of EGF, VEGF, and PCNA with cell proliferation roles.
本研究旨在评估食用燕窝（EBN）补充剂对暴露于醋酸铅（LA）毒性的大鼠子宫的保护作用。
设立五个治疗组如下：第1组（C）给予蒸馏水；第2组（T0）给予LA（10毫克/千克体重[BW]）；第3组（T1）、第4组（T2）和第5组（T3）分别给予LA（10毫克/千克BW）加不同浓度梯度的30、60和120毫克/千克BW的EBN。在第5周对大鼠实施安乐死，采集血液进行超氧化物歧化酶（SOD）测定，并采集子宫进行组织形态学研究以及表皮生长因子（EGF）、血管内皮生长因子（VEGF）和增殖细胞核抗原（PCNA）的表达分析。
结果显示，在未补充EBN的情况下，LA会导致暴露大鼠的子宫内膜细胞破坏和子宫腺坏死，而在EBN治疗组中损伤程度降低；T3组对LA毒性的改善作用最强，子宫腺数量也有所增加。与对照组相比，补充EBN的组中SOD水平也有所提高。免疫组织化学结果显示，与其他治疗组相比，T3组中EGF、VEGF和PCNA水平的表达显著更高（p<0.05）。EBN维持了抗氧化剂 - 活性氧平衡的上调。
研究结果表明，EBN可能通过增强酶促抗氧化剂（SOD）活性以及具有细胞增殖作用的EGF、VEGF和PCNA的表达，改善LA毒性对子宫的有害影响。
