Parsons J C, Bowman D D, Grieve R B
Am J Trop Med Hyg. 1986 Sep;35(5):974-81. doi: 10.4269/ajtmh.1986.35.974.
The distribution of excretory-secretory antigens of larval Toxocara canis (TEX) in tissue was studied in cases of both acute and chronic experimental murine toxocariasis. Liver samples were collected from BALB/c BYJ mice killed either 2 days (acute) or 8 months (chronic) after intragastric inoculation with infective T. canis eggs. Cryostat sections of liver were stained using biotin-conjugated, rabbit anti-TEX antibody with an avidin-fluorescein isothiocyanate indicator. In both acute and chronic infections larval sections were brightly fluorescent. The patterns of antigen deposition in acute infections were indicative of larval migration. In tissue sections from chronic infections, antigens were observed mainly within the core of granulomas centered around larvae and within the inner rim of the collagen capsule of granulomas in which a larva or larval remnant was not detected. When present within the collagenous capsule, TEX appeared to be extracellular. These findings suggest that an accurate histopathological diagnosis of larval toxocariasis may be possible in biopsy or autopsy material lacking an obvious etiological agent.
在急性和慢性实验性小鼠弓首蛔虫病病例中，研究了犬弓首线虫幼虫排泄-分泌抗原（TEX）在组织中的分布。从经口接种感染性犬弓首线虫卵后2天（急性）或8个月（慢性）处死的BALB/c BYJ小鼠收集肝脏样本。肝脏的低温切片用生物素偶联的兔抗TEX抗体和抗生物素蛋白-异硫氰酸荧光素指示剂染色。在急性和慢性感染中，幼虫切片均发出明亮荧光。急性感染中抗原沉积模式表明幼虫在迁移。在慢性感染的组织切片中，抗原主要在围绕幼虫的肉芽肿核心内以及未检测到幼虫或幼虫残体的肉芽肿胶原囊的内缘内观察到。当存在于胶原囊中时，TEX似乎位于细胞外。这些发现表明，在缺乏明显病原体的活检或尸检材料中，可能对幼虫弓首蛔虫病进行准确的组织病理学诊断。
