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DkmiR397 通过负向调控中国 PCNA 柿子中的原花青素生物合成。

DkmiR397 Regulates Proanthocyanidin Biosynthesis via Negative Modulating in Chinese PCNA Persimmon.

机构信息

Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.

出版信息

Int J Mol Sci. 2022 Mar 16;23(6):3200. doi: 10.3390/ijms23063200.

DOI:10.3390/ijms23063200
PMID:35328620
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8951489/
Abstract

Persimmon fruits accumulate a large amount of proanthocyanidins (PAs), which makes an astringent sensation. Proanthocyanidins (PAs) are the polymers of flavan-3-ols stored in plant vacuoles under laccase activation. A laccase gene, , is putatively involved in PAs biosynthesis and regulated by microRNA (DkmiR397) in persimmon. However, the polymerization of PAs in association with miRNA397 still needs to be explored in persimmon. Here, we identified pre-DkmiR397 and its target gene in 'Eshi 1' persimmon. Histochemical staining with GUS and dual luciferase assay both confirmed DkmiR397- binding after co-transformation in tobacco leaves. Diverse expression patterns of and DkmiR397 were exhibited during persimmon fruit development stages. Moreover, a contrasting expression pattern was also observed after the combined -miR397 transformation in persimmon leaves, suggesting that DkmiR397 might be a negative regulator of . Similarly, the transient transformation of DkmiR397 in persimmon fruit discs in vitro also reduced PA accumulation by repressing , whereas the up-regulation of increased the accumulation of PAs by short tandem target mimic STTM-miR397. A similar expression pattern was observed when overexpressing of in wild type (WT) and overexpression of , DkmiR397 in persimmon leaf callus. Our results revealed that the role of DkmiR397 repressed the expression of concerning PA biosynthesis, providing a potential target for the manipulation of PAs metabolism in persimmon.

摘要

柿子果实积累大量的原花青素（PAs），这使得它有一种涩味。原花青素（PAs）是在漆酶激活下储存在植物液泡中的黄烷-3-醇聚合物。一个漆酶基因， ，被认为参与了 PAs 的生物合成，并受柿子中的 microRNA（DkmiR397）调控。然而，在柿子中与 miRNA397 相关的 PAs 聚合仍需要进一步研究。在这里，我们在 'Eshi 1' 柿子中鉴定了 pre-DkmiR397 及其靶基因 。GUS 组织化学染色和双荧光素酶报告基因实验均证实了 DkmiR397 在烟草叶片共转化后的结合。在柿子果实发育阶段， 和 DkmiR397 的表达模式存在差异。此外，在柿子叶片中进行 -miR397 联合转化后，也观察到了相反的表达模式，这表明 DkmiR397 可能是 的负调控因子。同样，在体外瞬时转化 DkmiR397 也减少了 PA 的积累，抑制了 ，而通过短串联靶模拟物 STTM-miR397 上调 则增加了 PAs 的积累。在过表达 野生型（WT）和过表达 ，DkmiR397 在柿子叶愈伤组织中的瞬时转化中也观察到了类似的表达模式。我们的结果表明，DkmiR397 抑制了与 PA 生物合成有关的 表达，为操纵柿子中 PAs 代谢提供了一个潜在的靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba9a/8951489/499434362ddf/ijms-23-03200-g007.jpg
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Plant Sci. 2020 Mar;292:110390. doi: 10.1016/j.plantsci.2019.110390. Epub 2019 Dec 27.
3
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4
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