Lin Baoli, Ma He, Zhang Kezhong, Cui Jinteng
College of Landscape Architecture, Beijing University of Agriculture, Beijing, China.
Laboratory of Urban and Rural Ecological Environment, Beijing University of Agriculture, Beijing, China.
Front Plant Sci. 2023 Jan 12;13:1047452. doi: 10.3389/fpls.2022.1047452. eCollection 2022.
is a colorful tree species with considerable ornamental and economic value. However, little is known about the metabolism and regulatory mechanism of leaf color change in . To reveal the molecular mechanism of leaf color change in , the present study examined the bud mutation branches and compared the metabolites of the red leaves (AR) of the bud mutation branches of with those of the green leaves (AG) of the wild-type branches. It was found that the chlorophyll and carotenoids content of the red leaves decreased significantly, while anthocyanins, and various antioxidant enzymes increased significantly compared with the green leaves. The glycosides cyanidin, pelargonidin, malvidin, petunidin, delphinidin, and peonidin were detected in AR by liquid chromatography-mass spectrometry. The cyanidin glycosides increased, and cyanidin 3-O-glycoside was significantly upregulated. We analyzed the transcriptome and small RNA of leaves and detected 4061 differentially expressed mRNAs and 116 differentially expressed miRNAs. Through miRNA-mRNA association analysis, five differentially expressed modules were found; one miRNA targeted three genes, and four miRNAs targeted a single gene. Among them, miR160b, miR6300, and miR396g were found to be the key miRNAs regulating stable anthocyanin accumulation in leaves. By revealing the physiological response of leaf color change and the molecular regulatory mechanism of the miRNA, this study provides new insight into the molecular regulatory mechanism of leaf color change, thereby offering a foundation for future studies.
是一种具有较高观赏和经济价值的彩色树种。然而，关于其叶片颜色变化的代谢及调控机制却知之甚少。为揭示其叶片颜色变化的分子机制，本研究对芽变枝条进行了检测，并比较了芽变枝条红叶（AR）与野生型枝条绿叶（AG）的代谢产物。结果发现，与绿叶相比，红叶的叶绿素和类胡萝卜素含量显著降低，而花青素及多种抗氧化酶则显著增加。通过液相色谱 - 质谱联用在AR中检测到了矢车菊素、天竺葵素、锦葵素、矮牵牛素、飞燕草素和芍药色素糖苷。矢车菊素糖苷增加，且矢车菊素3 - O - 糖苷显著上调。我们分析了其叶片的转录组和小RNA，检测到4061个差异表达的mRNA和116个差异表达的miRNA。通过miRNA - mRNA关联分析，发现了5个差异表达模块；1个miRNA靶向3个基因，4个miRNA各靶向1个基因。其中，miR160b、miR6300和miR396g被发现是调控其叶片中稳定花青素积累的关键miRNA。本研究通过揭示叶片颜色变化的生理响应及miRNA的分子调控机制，为叶片颜色变化的分子调控机制提供了新的见解，从而为未来的研究奠定了基础。
