Netherlands Institute for Neuroscience Amsterdam, 1105 BA Amsterdam, The Netherlands.
Department of Biomedical Physics, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
Int J Mol Sci. 2022 Mar 16;23(6):3202. doi: 10.3390/ijms23063202.
Patients with congenital nystagmus, involuntary eye movements, often have a reduced visual acuity. Some of these patients have a retinal-specific mutation in the protein nyctalopin, which is also present in the mouse. In these mice, retinal ganglion cells (RGCs) have oscillatory activity, which leads to expanded axonal projections towards the dLGN and consequently to a desegregation of retinal projections to the brain. In this study, we investigate whether the receptive fields of RGCs have also expanded by measuring the size of their receptive fields using MEA recordings. Contrary to our expectation, relative to wild-type (WT) mice we found receptive field sizes in the retina had not increased but instead had decreased for green-light preferring RGCs. Additionally, we also found the receptive fields of UV-light preferring RGCs are larger than green-light preferring RGCs in both WT and mice.
患有先天性眼球震颤（一种不自主的眼球运动）的患者通常视力会下降。这些患者中，有一些人的蛋白 nyctalopin 存在视网膜特异性突变，而该蛋白在 鼠中也存在。在这些鼠中，视网膜神经节细胞（RGCs）具有振荡活性，这导致其轴突向外侧膝状体的投射扩展，从而导致视网膜投射到大脑的分离。在这项研究中，我们通过使用 MEAs 记录来测量其感受野的大小，来研究 RGCs 的感受野是否也已经扩大。与我们的预期相反，与野生型（WT）鼠相比，我们发现绿光偏好型 RGCs 的感受野大小没有增加，反而减少了。此外，我们还发现，在 WT 和 鼠中，UV 光偏好型 RGCs 的感受野都大于绿光偏好型 RGCs。
