Mak Siew Thong, Leong Xin Fang, Tew In Meei, Kumolosasi Endang, Wong Lishen
Department of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia.
Department of Craniofacial Diagnostics and Biosciences, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia.
Materials (Basel). 2022 Mar 8;15(6):2012. doi: 10.3390/ma15062012.
This study aimed to investigate the antibacterial activity of three endodontic sealers, AH Plus, iRoot SP, and EndoSeal MTA, against four planktonic bacteria species. The antibacterial activity of the three endodontic sealers was assessed using a modified direct contact test. Bacteria suspension of , , and were left in contact with the sealers that were pre-set or set for 1, 3, 7, and l4 days for an hour. Freshly mixed AH Plus and EndoSeal MTA were highly effective against all four tested bacteria as no surviving bacteria were recovered after treatment. Meanwhile, freshly mixed iRoot SP was not able to kill all bacteria, regardless of the species, demonstrating a weak antibacterial effect. After 24 h, AH Plus lost its antibacterial activity. EndoSeal MTA showed a strong and extended bactericidal effect against and for 3 days and for 7 days. In conclusion, fresh AH Plus and EndoSeal MTA exhibited a potent effect against all four bacteria species. EndoSeal MTA remained effective after setting when tested against , and . Among all tested sealers, iRoot SP demonstrates the weakest antibacterial activity.
本研究旨在调查三种根管封闭剂AH Plus、iRoot SP和EndoSeal MTA对四种浮游细菌的抗菌活性。采用改良的直接接触试验评估这三种根管封闭剂的抗菌活性。将金黄色葡萄球菌、大肠杆菌、粪肠球菌和白色念珠菌的细菌悬液与预先设定或设定1、3、7和14天的封闭剂接触1小时。新鲜混合的AH Plus和EndoSeal MTA对所有四种受试细菌都非常有效，因为处理后未检测到存活细菌。同时，无论细菌种类如何，新鲜混合的iRoot SP都不能杀死所有细菌，显示出较弱的抗菌效果。24小时后，AH Plus失去了抗菌活性。EndoSeal MTA对金黄色葡萄球菌和大肠杆菌显示出强烈且持久的杀菌作用，持续3天，对粪肠球菌持续7天。总之，新鲜的AH Plus和EndoSeal MTA对所有四种细菌都有强效作用。EndoSeal MTA在凝固后对金黄色葡萄球菌、大肠杆菌和粪肠球菌进行测试时仍然有效。在所有测试的封闭剂中，iRoot SP的抗菌活性最弱。
