Li Jun-Nan, Yi He-Nan, Yang Fang-Nan, Miao Li, Du Qi-Tao, Du Xiao-Pei
School of Stomatology, Dalian Medical University Dalian 116021, Liaoning, China.
Department of Stomatology, Xinhua Hospital, Dalian University Dalian 116021, Liaoning, China.
Am J Transl Res. 2025 Jun 15;17(6):4375-4386. doi: 10.62347/WAIK7340. eCollection 2025.
To evaluate and compare the sealing efficacy of three root canal filling techniques: AH plus with warm vertical compaction (WVC), iRoot SP with the single-cone technique, and ultrasonic-assisted iRoot SP single-cone technique.
Ninety premolars extracted for orthodontic purposes at Dalian Stomatological Hospital between March 2022 and March 2024 were collected. After standardized root canal preparation, specimens were randomly assigned to three groups: AH plus group (WVC technique + AH plus), iRoot SP group (single-cone + iRoot SP), and US-iRoot SP group (ultrasonic-assisted single-cone + iRoot SP). Apical microleakage was assessed using dye leakage tests. Sealer penetration at 3 mm, 5 mm, and 8 mm from the apex was evaluated using optical microscopy and confocal laser scanning microscopy (CLSM). Stereomicroscopy and scanning electron microscopy (SEM) were used to analyze sealer distribution within the root canal.
The US-iRoot SP group exhibited significantly less apical microleakage than the AH plus and iRoot SP groups (P<0.05). CLSM analysis revealed significantly greater average and maximum sealer penetration depths in the US-iRoot SP group at all evaluated levels (P<0.05). Stereomicroscopy showed the smallest gap between sealer and gutta-percha in the US-iRoot SP group. SEM confirmed improved adaptation of the sealer to the dentin wall and deeper penetration into dentinal tubules.
The ultrasonic-assisted iRoot SP single-cone technique significantly enhances root canal obturation quality compared to both the conventional iRoot SP single-cone technique and the AH plus with WVC technique.
评估并比较三种根管充填技术的封闭效果：AH plus热垂直加压技术、iRoot SP单锥技术以及超声辅助iRoot SP单锥技术。
收集2022年3月至2024年3月期间在大连口腔医院因正畸目的拔除的90颗前磨牙。经过标准化的根管预备后，将标本随机分为三组：AH plus组（热垂直加压技术 + AH plus）、iRoot SP组（单锥技术 + iRoot SP）和US-iRoot SP组（超声辅助单锥技术 + iRoot SP）。使用染料渗漏试验评估根尖微渗漏情况。利用光学显微镜和共聚焦激光扫描显微镜（CLSM）评估根尖3 mm、5 mm和8 mm处封闭剂的渗透情况。使用体视显微镜和扫描电子显微镜（SEM）分析根管内封闭剂的分布情况。
US-iRoot SP组的根尖微渗漏显著少于AH plus组和iRoot SP组（P<0.05）。CLSM分析显示，在所有评估水平上，US-iRoot SP组的封闭剂平均渗透深度和最大渗透深度均显著更大（P<0.05）。体视显微镜显示US-iRoot SP组中封闭剂与牙胶之间的间隙最小。SEM证实封闭剂与牙本质壁的贴合度提高，且向牙本质小管内的渗透更深。
与传统的iRoot SP单锥技术和AH plus热垂直加压技术相比，超声辅助iRoot SP单锥技术显著提高了根管充填质量。
