Kobayashi S, Suzuki M, Endo T, Tsuji S, Daniel E E
Arch Histol Jpn. 1986 Jun;49(2):159-88.
Immunostained sections and whole-mount preparations of the layers of the guinea pig jejunum were investigated by an improved peroxidase-antiperoxidase method using an antiserum to S-100 protein. A delicate latticework of S-100 protein immunopositive glial cells was demonstrated extending in the longitudinal muscle layer, myenteric or Auerbach's plexus, circular muscle layer including the deep muscular plexus, submucous layer including the submucous or Meissner's plexus, lamina muscularis mucosae and lamina propria mucosae. The whole enteric nerve plexuses consisted of two subsystems; nerve plexuses of the muscular coat and those of the submucous and mucous coats. These two subsystems were joined to each other by thick, connecting branches perforating the inner circular muscle layer. Extrinsic nerves entering the myenteric plexus formed a specialized junctional structure containing S-100 protein immunopositive glial cells, whereas those entering the submucous plexus ran along the submucous arteries. We proposed the term enteroglial cells to designate the S-100 protein immunopositive cells which ensheathed the somata and processes of the enteric neurons. The frameworks of all structures in the enteric nerve plexuses from the largest ganglia to the thinnest nerve fasciculi were constructed of these enteroglial cells. A spectrum of the enteroglial cells was presented. Those in the myenteric and submucous ganglia were found similar to the astroglia of the central nervous system and to the satellite cells in the peripheral ganglia. Those in the primary and secondary fasciculi of the myenteric plexus formed a kind of neuropil together with the neuronal processes. Those in the tertiary fasciculi of the muscular coat formed the framework of the autonomic ground plexus. We tentatively concluded that the interstitial cells of Cajal contain an immunoreactivity for S-100 protein, and thus are glial in nature. The occurrence of specialized enteroglial cells with a neuron-like function was discussed in the autonomic ground plexus of the muscular coat. In the lamina propria mucosae, there was a fine latticework of the S-100 protein immunopositive enteroglial cells. This latticework corresponded to that of the interstitial cells of Cajal in the villous and periglandular plexuses.
使用抗S - 100蛋白抗血清，通过改进的过氧化物酶 - 抗过氧化物酶方法研究豚鼠空肠各层的免疫染色切片和整装标本。结果显示，S - 100蛋白免疫阳性的神经胶质细胞形成了精细的网络结构，延伸至纵肌层、肌间或奥尔巴赫神经丛、包括深肌丛的环肌层、包括黏膜下或迈斯纳神经丛的黏膜下层、黏膜肌层和固有层。整个肠神经丛由两个子系统组成；肌层神经丛和黏膜下及黏膜层神经丛。这两个子系统通过穿过内环肌层的粗大连接分支相互连接。进入肌间神经丛的外在神经形成了一种特殊的连接结构，其中含有S - 100蛋白免疫阳性的神经胶质细胞，而进入黏膜下神经丛的外在神经则沿着黏膜下动脉走行。我们提出“肠神经胶质细胞”这一术语来指代包裹肠神经元胞体和突起的S - 100蛋白免疫阳性细胞。从最大的神经节到最细的神经束，肠神经丛中所有结构的框架均由这些肠神经胶质细胞构成。展示了一系列肠神经胶质细胞。发现肌间和黏膜下神经节中的肠神经胶质细胞类似于中枢神经系统的星形胶质细胞和外周神经节中的卫星细胞。肌间神经丛初级和次级神经束中的肠神经胶质细胞与神经元突起一起形成了一种神经毡。肌层三级神经束中的肠神经胶质细胞形成了自主神经基层丛的框架。我们初步得出结论， Cajal间质细胞含有对S - 100蛋白的免疫反应性，因此本质上是神经胶质细胞。在肌层的自主神经基层丛中讨论了具有神经元样功能的特殊肠神经胶质细胞的存在。在固有层中，存在S - 100蛋白免疫阳性肠神经胶质细胞的精细网络结构。该网络结构与绒毛和腺周丛中Cajal间质细胞的网络结构相对应。
