Anderson C R, Edwards S L
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Australia.
Histochemistry. 1993 Dec;100(6):457-64. doi: 10.1007/BF00267826.
Cholera toxin subunit b was found in vivo and in vitro to label interstitial cells of Cajal in the intestine of rat and mouse. Cholera toxin-labelled interstitial cells were present in the subserosa, the myenteric plexus and the deep muscular plexus of mouse small intestine, and the deep muscular plexus only of the rat small intestine. In the large intestine of the mouse, interstitial cells were present in the subserosa and in a plexus associated with the inner surface of the circular muscle, while in the rat they were only present in the latter location. Macrophages, which were present in many of the same locations as interstitial cells, were also labelled by cholera toxin but could be distinguished from interstitial cells by their ability to take-up fluorescein isothiocyanate-labelled dextran. Labelling with subunit b of cholera toxin is a simple way of labelling interstitial cells of Cajal and which is compatible with a range of physiological and histological procedures.
体内和体外研究均发现，霍乱毒素B亚单位可标记大鼠和小鼠肠道中的 Cajal 间质细胞。霍乱毒素标记的间质细胞存在于小鼠小肠的浆膜下层、肌间神经丛和深肌丛，而在大鼠小肠中仅存在于深肌丛。在小鼠大肠中，间质细胞存在于浆膜下层以及与环肌内表面相关的神经丛中，而在大鼠中，它们仅存在于后一部位。巨噬细胞与间质细胞存在于许多相同位置，也可被霍乱毒素标记，但可通过其摄取异硫氰酸荧光素标记葡聚糖的能力与间质细胞区分开来。用霍乱毒素B亚单位进行标记是标记 Cajal 间质细胞的一种简单方法，且与一系列生理和组织学程序兼容。
