Fehér Enikő, Kaszab Eszter, Bali Krisztina, Hoitsy Márton, Sós Endre, Bányai Krisztián
Veterinary Medical Research Institute, H-1143 Budapest, Hungary.
Conservation and Veterinary Services, Budapest Zoo and Botanical Garden, H-1164 Budapest, Hungary.
Life (Basel). 2022 Mar 3;12(3):368. doi: 10.3390/life12030368.
Circoviruses occur in a variety of animal species and are common pathogens of mammalian and avian hosts. In our study internal organ samples of wild birds were processed for screening of circoviral sequences. Two novel viruses were identified and characterized in specimens of a little bittern and a European bee-eater that suffered from wing injuries, were weakened, had liver or kidney failures, and finally succumbed at a rescue station. The 1935 nt and 1960 nt long viral DNA genomes exhibited a genomic structure typical for circoviruses and were predicted to encode replication-associated protein in the viral strand, and a capsid protein in the complementary strand of the replicative intermediate DNA form. The genome of the newly described viruses showed 37.6% pairwise identity with each other and ≤41.5% identity with circovirus sequences, and shared a common branch with fish, human and Weddel seal circoviruses in the phylogenetic tree, implying evolutionary relationship among the ancestors of these viruses. Based on the results the little bittern and European bee-eater circoviruses represent two distinct species of the genus, family.
圆环病毒存在于多种动物物种中，是哺乳动物和鸟类宿主的常见病原体。在我们的研究中，对野生鸟类的内脏样本进行了处理，以筛选圆环病毒序列。在一只小苇鳽和一只欧洲蜂虎的样本中鉴定并表征了两种新型病毒，这两只鸟翅膀受伤、身体虚弱、出现肝或肾衰竭，最终在一个救助站死亡。长度为1935个核苷酸和1960个核苷酸的病毒DNA基因组呈现出圆环病毒典型的基因组结构，预计在病毒链中编码复制相关蛋白，在复制中间体DNA形式的互补链中编码衣壳蛋白。新描述病毒的基因组彼此之间显示出37.6%的成对同一性，与圆环病毒序列的同一性≤41.5%，并且在系统发育树中与鱼类、人类和威德尔海豹圆环病毒共享一个共同分支，这意味着这些病毒的祖先之间存在进化关系。基于这些结果，小苇鳽和欧洲蜂虎圆环病毒代表了该属、该科的两个不同物种。
