Sartori Rafaela Basso, Vendruscolo Raquel Guidetti, Ribeiro Stephanie Reis, Furlan Valcenir Júnior Mendes, Wagner Roger, Zepka Leila Queiroz, Jacob-Lopes Eduardo
Bioprocess Intensification Group, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, RS, Brazil.
Food Science and Technology, Federal University of Pampa (UNIPAMPA), Itaqui 97650-000, RS, Brazil.
Life (Basel). 2022 Mar 21;12(3):462. doi: 10.3390/life12030462.
The objective of this work was to evaluate the effect of different photo-cycles on the growth and biochemical profile of CPCC05, focusing on food interest compounds. The photo-cycle conditions were separated into three groups: long-term photo-cycles (24:0, 22:2, 20:4, 18:6, 12:12, and 10:14 (h:h)), frequency photo-cycles (2, 4, 8, 12, 24, and 48 times per day (t/d)), and short photo-cycles (0.91:0.09, 0.83:0.17, 0.75:0.25, and 0.50:0.50 (s:s)) of light:dark, respectively. The results showed these microalgae can store enough energy to support cell growth for continuous periods of up to 2 h in the dark, without affecting the productivity of the process. This 2 h, when divided into 2 cycles per day (2 t/d), showed the best growth condition (3700 mg L), generation time (14.40 h), and maximum biomass productivity (21.43 mg L h). This photo-cycle of 2 t/d was also the best condition for the production of total sterols. However, the values of polyunsaturated fatty acids, lipid content, and amino acids obtained higher yields in the short photo-cycle of 0.75:0.25. Thus, the modulation of light cycles becomes an important tool for boosting and directing the production of target molecules in phototrophic cultures of microalgae.
这项工作的目的是评估不同光周期对CPCC05生长和生化特性的影响，重点关注具有食品价值的化合物。光周期条件分为三组：长期光周期（24:0、22:2、20:4、18:6、12:12和10:14（小时:小时））、频率光周期（每天2、4、8、12、24和48次（次/天））以及短光周期（0.91:0.09、0.83:0.17、0.75:0.25和0.50:0.50（秒:秒））的光:暗周期。结果表明，这些微藻能够储存足够的能量，以支持细胞在黑暗中持续长达2小时的生长，而不影响该过程的生产力。当每天分为2个周期（2次/天）时，这2小时显示出最佳的生长条件（3700毫克/升）、世代时间（14.40小时）和最大生物量生产力（21.43毫克/升·小时）。这种2次/天的光周期也是总甾醇产量最高的条件。然而，多不饱和脂肪酸、脂质含量和氨基酸的产量在0.75:0.25的短光周期中更高。因此，光周期的调节成为提高和指导微藻光合培养中目标分子产量的重要工具。
