Setiawan Andi, Setiawan Fendi, Juliasih Ni Luh Gede Ratna, Widyastuti Widyastuti, Laila Aspita, Setiawan Wawan A, Djailani Fernandy M, Mulyono Mulyono, Hendri John, Arai Masayoshi
Department of Chemistry, Faculty of Mathematics and Natural Science, Lampung University, Bandar Lampung 35145, Indonesia.
Department of Biology, Faculty of Mathematics and Natural Science, Lampung University, Bandar Lampung 35145, Indonesia.
J Fungi (Basel). 2022 Mar 9;8(3):280. doi: 10.3390/jof8030280.
Secondary metabolites of actinomycetes are a potential source of bioactive compounds in the agricultural sector. This study aimed to determine the fungicidal properties of extracts of marine organism-derived actinomycetes. Actinomycetes were isolated from marine organisms using agar media with 1% colloidal chitin in artificial seawater. Then, the isolates were cultured on liquid media with 1% colloidal chitin in artificial seawater under static conditions for 14 days. The culture was extracted, the fungicide properties were evaluated using the microtiter 96-well plate method, and the influence of inhibition was visualized using apotome and SEM. Finally, the active extract was analyzed using LCMSMS. In the present study, 19 actinomycetes were isolated from marine organisms, and the isolates were examined with regard to their antifungal activities. Of these nineteen isolates, the isolate 19C38A1 was picked out from the rest. Hence, it showed significant control towards . The prospective strain 19C38A1 was determined to be 19C38A1. The extract 19C38A1 was shown to cause damage to cell integrity, indicated by the shrinking form, and inhibited germination in the ; subsequently, the chemical characteristics of the compound produced by the potential isolate 19C38A1 indicated the presence of benzimidazole compounds in the active fraction of C38BK2FA. These results indicate that actinomycetes derived from marine organisms near the coast of Oluhuta, Tomini Bay, Gorontalo, related to strain 19C38A1, are not widely known as sources of valuable fungicides. This preliminary information is important, as it can be used as a basis for further development in the search for fungicides derived from marine actinomycetes.
放线菌的次生代谢产物是农业领域生物活性化合物的潜在来源。本研究旨在确定海洋生物源放线菌提取物的杀菌特性。使用含1%胶体几丁质的琼脂培养基在人工海水中从海洋生物中分离放线菌。然后，将分离物在含1%胶体几丁质的人工海水液体培养基中在静态条件下培养14天。对培养物进行提取，使用96孔微量滴定板法评估杀菌剂特性，并使用光学切片显微镜和扫描电子显微镜观察抑制作用的影响。最后，使用液相色谱串联质谱对活性提取物进行分析。在本研究中，从海洋生物中分离出19株放线菌，并对分离物的抗真菌活性进行了检测。在这19个分离物中，分离物19C38A1脱颖而出。因此，它对……表现出显著的防治效果。确定潜在菌株为19C38A1。提取物19C38A1显示会破坏细胞完整性，表现为细胞萎缩，并抑制……中的萌发；随后，潜在分离物19C38A1产生的化合物的化学特性表明在C38BK2FA的活性部分中存在苯并咪唑化合物。这些结果表明，来自哥伦打洛省托米尼湾奥卢胡塔海岸附近海洋生物的与菌株19C38A1相关的放线菌，作为有价值杀菌剂的来源并不广为人知。这一初步信息很重要，因为它可作为进一步开发海洋放线菌源杀菌剂的基础。
