Bojanović Mila, Ignjatović Aleksandra, Stalević Marko, Arsić-Arsenijević Valentina, Ranđelović Marina, Gerginić Vladimir, Stojanović-Radić Zorica, Stojković Ognjen, Živković-Marinkov Emilija, Otašević Suzana
Medical Faculty, University of Niš, 18000 Niš, Serbia.
Clinic of Otorhinolaryngology, University Clinical Center Niš, 18000 Niš, Serbia.
J Fungi (Basel). 2022 Mar 18;8(3):315. doi: 10.3390/jof8030315.
Species of () complex and complex are predominant molds that are causative agents of otomycoses. The goal of this study was to investigate the clinical presentation, diagnostic procedure, and appearance of relapse in patients with -otomycosis, as well as to determine the biofilm production ability of species isolated in relapse. Thirty patients with laboratory evidenced -otomycosis followed by two check-ups (30 and 60 days after initiation of treatment with antimycotics for local application) were included in the study. For isolation and identification of spp. the standard mycological procedure was applied. Results showed very high sensitivity of microscopy, but 16.7% species required the optimal temperature of 27-28 °C for cultivation. Applied statistical cluster analysis showed a defined specific cluster/group of patients with complex-otomycosis. Sixty days after diagnosis and treatment initiation, six patients had a relapse, with the same species of genus being the cause. To establish the ability of biofilm production, the modified method described by Pierce and Kvasničková was performed, and all six species isolated in the relapse episode had the ability to produce biofilm. Official criteria and recommendations are needed due to the possibility of misdiagnosis, which leads to the prolongation and complication of the disease.
（）复合体和复合体的物种是耳真菌病的主要致病霉菌。本研究的目的是调查耳真菌病患者的临床表现、诊断程序和复发情况，以及确定复发时分离出的物种的生物膜形成能力。30例经实验室证实患有耳真菌病并随后进行两次检查（局部应用抗真菌药物治疗开始后30天和60天）的患者被纳入研究。对于分离和鉴定 spp.，采用了标准的真菌学程序。结果显示显微镜检查的敏感性非常高，但16.7%的物种需要27 - 28°C的最佳温度进行培养。应用统计聚类分析显示了一组明确的患有复合体耳真菌病的特定患者群。诊断和治疗开始60天后，6例患者复发，病因是同一属的物种。为确定生物膜形成能力，采用了Pierce和Kvasničková描述的改良方法，复发期分离出的所有6个物种都有形成生物膜的能力。由于存在误诊的可能性，这会导致疾病的延长和并发症，因此需要官方标准和建议。
