Department of Public Health and Infectious Diseases, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy.
Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy.
Molecules. 2022 Aug 25;27(17):5424. doi: 10.3390/molecules27175424.
Poly-(lactic--glycolic) acid (PLGA) is a biodegradable, biosafe, and biocompatible copolymer. The section causes otomycosis localized in the external auditory canal. In this research, , a species belonging to the section, was tested. Coumarin 6 and pterostilbene loaded in poly-(lactic--glycolic) acid nanoparticles (PLGA-coumarin6-NPs and PLGA-PTB-NPs) were tested for fungal cell uptake and antifungal ability against biofilm, respectively. Moreover, the activity of PLGA-PTB-NPs in inhibiting the infection was tested using larvae. The results showed a fluorescence signal, after 50 nm PLGA-coumarin6-NPs treatment, inside hyphae and along the entire thickness of the biofilm matrix, which was indicative of an efficient NP uptake. Regarding antifungal activity, a reduction in biofilm formation and mature biofilm with PLGA-PTB-NPs has been demonstrated. Moreover, in vivo experiments showed a significant reduction in mortality of infected larvae after injection of PLGA-PTB-NPs compared to free PTB at the same concentration. In conclusion, the PLGA-NPs system can increase the bioavailability of PTB in section biofilm by overcoming the biofilm matrix barrier and delivering PTB to fungal cells.
聚（乳酸-乙醇酸）（PLGA）是一种可生物降解、生物安全和生物相容的共聚物。该节导致外耳道局部发生真菌病。在这项研究中，测试了属于该节的一种真菌。分别用载香豆素 6 和紫檀芪的聚（乳酸-乙醇酸）纳米粒（PLGA-香豆素 6-NPs 和 PLGA-PTB-NPs）测试了真菌细胞摄取和抗真菌生物膜能力。此外，还用幼虫测试了 PLGA-PTB-NPs 抑制感染的活性。结果显示，在 50nm PLGA-香豆素 6-NPs 处理后，在菌丝内部和整个生物膜基质中都有荧光信号，这表明 NP 被有效摄取。关于抗真菌活性，已经证明 PLGA-PTB-NPs 减少了生物膜的形成和成熟生物膜。此外，体内实验显示，与相同浓度的游离紫檀芪相比，注射 PLGA-PTB-NPs 后感染幼虫的死亡率显著降低。总之，PLGA-NPs 系统可以通过克服生物膜基质屏障并将紫檀芪递送到真菌细胞中来增加紫檀芪在节段生物膜中的生物利用度。
