Lin Yong-Wang, Jing Mei-Ling, Li Yu-Qing, Zhou Xue-Dong
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2022 Mar;53(2):268-273. doi: 10.12182/20220360205.
To explore the effects of nicotinamide (NAM) on the growth, biofilm formation and exopolysaccharides (EPS) production of .
The minimum inhibitory concentration (MIC) of NAM on was determined by the planktonic bacterial susceptibility assay. The NAM mass concentrations were set as 1/2 MIC, 1/4 MIC and 1/8 MIC for hree separate treatment groups. Culture medium without NAM was used in the negative control group and culture medium containing 0.1 mg/mL NaF was used for the positive control group (except for the scanning electron microscopy). The growth curves of under different NAM concentrations were drawn. Crystal violet assay and anthrone-sulfuric acid method were used to explore the effects of NAM on biofilm formation and water-insoluble EPS production, respectively. The morphology and structure of planktons and biofilms after NAM treatment were observed by scanning electron microscopy.
The MIC of NAM on was 32 μg/μL. After 16 μg/μL (1/2 MIC), 8 μg/μL (1/4 MIC) and 4 μg/μL (1/8 MIC) NAM treatments, growth and biofilm formation were inhibited, with the 16 μg/μL NAM group displaying the most significant inhibitory effects. The synthesis of EPS decreased significantly in the 16 μg/μL and 8 μg/μL NAM groups in comparison with that of the negative control group (<0.05). Under scanning electron microscope, the cell length of was shortened, the cell width was extended, and the length/width ratio was decreased, showing significant difference when comparing the 16 μg/μL and 8 μg/μL NAM groups with the negative control group (<0.05).
Under the influence of NAM at certain concenrations, the growth, biofilm formation, and EPS synthesis of were inhibited.
探讨烟酰胺（NAM）对[具体细菌名称未给出]生长、生物膜形成及胞外多糖（EPS）产生的影响。
采用浮游菌药敏试验测定NAM对[具体细菌名称未给出]的最低抑菌浓度（MIC）。将NAM质量浓度设置为1/2 MIC、1/4 MIC和1/8 MIC，分为三个单独的处理组。阴性对照组使用不含NAM的培养基，阳性对照组（扫描电子显微镜观察除外）使用含0.1 mg/mL NaF的培养基。绘制不同NAM浓度下[具体细菌名称未给出]的生长曲线。分别采用结晶紫法和蒽酮 - 硫酸法探讨NAM对[具体细菌名称未给出]生物膜形成及水不溶性EPS产生的影响。通过扫描电子显微镜观察NAM处理后浮游菌和生物膜的形态与结构。
NAM对[具体细菌名称未给出]的MIC为32 μg/μL。经16 μg/μL（1/2 MIC）、8 μg/μL（1/4 MIC）和4 μg/μL（1/8 MIC）NAM处理后，[具体细菌名称未给出]的生长和生物膜形成受到抑制，其中16 μg/μL NAM组的抑制作用最为显著。与阴性对照组相比，16 μg/μL和8 μg/μL NAM组中EPS合成显著减少（P<0.05）。在扫描电子显微镜下，[具体细菌名称未给出]的细胞长度缩短且细胞宽度变宽，长宽比降低，16 μg/μL和8 μg/μL NAM组与阴性对照组相比差异显著（P<0.05）。
在一定浓度的NAM影响下[具体细菌名称未给出]的生长、生物膜形成及EPS合成受到抑制。
